|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM312131208 |
| 003 |
DE-627 |
| 005 |
20250227133956.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.2144/btn-2020-0078
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1040.xml
|
| 035 |
|
|
|a (DE-627)NLM312131208
|
| 035 |
|
|
|a (NLM)32635743
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Zhang, Yinhua
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 24.09.2020
|
| 500 |
|
|
|a Date Revised 10.12.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated techniques and enabling accurate, high-throughput diagnostics
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a LAMP
|
| 650 |
|
4 |
|a PCR
|
| 650 |
|
4 |
|a SARS-CoV-2
|
| 650 |
|
4 |
|a high-throughput screening
|
| 650 |
|
4 |
|a isothermal amplification
|
| 650 |
|
4 |
|a molecular diagnostics
|
| 650 |
|
7 |
|a Phenolsulfonphthalein
|2 NLM
|
| 650 |
|
7 |
|a I6G9Y0J1OJ
|2 NLM
|
| 650 |
|
7 |
|a Guanidine
|2 NLM
|
| 650 |
|
7 |
|a JU58VJ6Y3B
|2 NLM
|
| 700 |
1 |
|
|a Ren, Guoping
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Buss, Jackson
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Barry, Andrew J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Patton, Gregory C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Tanner, Nathan A
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 69(2020), 3 vom: 01. Sept., Seite 178-185
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
|
| 773 |
1 |
8 |
|g volume:69
|g year:2020
|g number:3
|g day:01
|g month:09
|g pages:178-185
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.2144/btn-2020-0078
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_21
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_39
|
| 912 |
|
|
|a GBV_ILN_40
|
| 912 |
|
|
|a GBV_ILN_50
|
| 912 |
|
|
|a GBV_ILN_60
|
| 912 |
|
|
|a GBV_ILN_62
|
| 912 |
|
|
|a GBV_ILN_65
|
| 912 |
|
|
|a GBV_ILN_70
|
| 912 |
|
|
|a GBV_ILN_99
|
| 912 |
|
|
|a GBV_ILN_121
|
| 912 |
|
|
|a GBV_ILN_130
|
| 912 |
|
|
|a GBV_ILN_227
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_618
|
| 912 |
|
|
|a GBV_ILN_640
|
| 912 |
|
|
|a GBV_ILN_754
|
| 912 |
|
|
|a GBV_ILN_2001
|
| 912 |
|
|
|a GBV_ILN_2002
|
| 912 |
|
|
|a GBV_ILN_2003
|
| 912 |
|
|
|a GBV_ILN_2005
|
| 912 |
|
|
|a GBV_ILN_2006
|
| 912 |
|
|
|a GBV_ILN_2007
|
| 912 |
|
|
|a GBV_ILN_2008
|
| 912 |
|
|
|a GBV_ILN_2009
|
| 912 |
|
|
|a GBV_ILN_2010
|
| 912 |
|
|
|a GBV_ILN_2012
|
| 912 |
|
|
|a GBV_ILN_2015
|
| 912 |
|
|
|a GBV_ILN_2018
|
| 912 |
|
|
|a GBV_ILN_2023
|
| 912 |
|
|
|a GBV_ILN_2035
|
| 912 |
|
|
|a GBV_ILN_2040
|
| 912 |
|
|
|a GBV_ILN_2060
|
| 912 |
|
|
|a GBV_ILN_2099
|
| 912 |
|
|
|a GBV_ILN_2105
|
| 912 |
|
|
|a GBV_ILN_2121
|
| 912 |
|
|
|a GBV_ILN_2470
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 69
|j 2020
|e 3
|b 01
|c 09
|h 178-185
|