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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.0c01188
|2 doi
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|a pubmed24n1040.xml
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|a (DE-627)NLM312016018
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|a (NLM)32623897
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Liu, Yangmingyue
|e verfasserin
|4 aut
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| 245 |
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|a Creating Asymmetric Phospholipid Vesicles via Exchange With Lipid-Coated Silica Nanoparticles
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 22.09.2020
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|a Date Revised 06.08.2021
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Recently, effort has been placed into fabricating model free-floating asymmetric lipid membranes, such as asymmetric vesicles. Here, we report on the use of lipid-coated silica nanoparticles to exchange lipids with initially symmetric vesicles to generate composition-controlled asymmetric vesicles. Our method relies on the simple and natural exchange of lipids between membranes through an aqueous medium. Using a selected temperature, time, and ratio of lipid-coated silica nanoparticles to vesicles, we produced a desired highly asymmetric leaflet composition. At this point, the silica nanoparticles were removed by centrifugation, leaving the asymmetric vesicles in solution. In the present work, the asymmetric vesicles were composed of isotopically distinct dipalmitoylphosphatidylcholine lipids. Lipid asymmetry was detected by both small-angle neutron scattering (SANS) and proton nuclear magnetic resonance (1H NMR). The rate at which the membrane homogenizes at 75 °C was also assessed
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 700 |
1 |
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|a Kelley, Elizabeth G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Batchu, Krishna C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Porcar, Lionel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Perez-Salas, Ursula
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 36(2020), 30 vom: 04. Aug., Seite 8865-8873
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:36
|g year:2020
|g number:30
|g day:04
|g month:08
|g pages:8865-8873
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|u http://dx.doi.org/10.1021/acs.langmuir.0c01188
|3 Volltext
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|h 8865-8873
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