CDK8 is associated with RAP2.6 and SnRK2.6 and positively modulates abscisic acid signaling and drought response in Arabidopsis

CDK8 is associated with RAP2.6 and SnRK2.6 and positively modulates abscisic acid signaling and drought response in Arabidopsis

© 2020 The Authors. New Phytologist © 2020 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 228(2020), 5 vom: 15. Dez., Seite 1573-1590
1. Verfasser: Zhu, Yingfang (VerfasserIn)
Weitere Verfasser: Huang, Pengcheng, Guo, Pengcheng, Chong, Leelyn, Yu, Gaobo, Sun, Xiaoli, Hu, Tao, Li, Yuan, Hsu, Chuan-Chih, Tang, Kai, Zhou, Yun, Zhao, Chunzhao, Gao, Wei, Tao, W Andy, Mengiste, Tesfaye, Zhu, Jian-Kang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ABA-responsive genes Arabidopsis CDK8 Mediator complex RAP2.6 SnRK2.6 abiotic stress responses Arabidopsis Proteins mehr... RAP2.6 protein, Arabidopsis SnRK2 protein, Arabidopsis Transcription Factors Abscisic Acid 72S9A8J5GW Protein Serine-Threonine Kinases EC 2.7.11.1 CDK8 protein, Arabidopsis EC 2.7.11.22 Cyclin-Dependent Kinase 8
Beschreibung
Zusammenfassung:© 2020 The Authors. New Phytologist © 2020 New Phytologist Trust.
CDK8 is a key subunit of Mediator complex, a large multiprotein complex that is a fundamental part of the conserved eukaryotic transcriptional machinery. However, the biological functions of CDK8 in plant abiotic stress responses remain largely unexplored. Here, we demonstrated CDK8 as a critical regulator in the abscisic acid (ABA) signaling and drought response pathways in Arabidopsis. Compared to wild-type, cdk8 mutants showed reduced sensitivity to ABA, impaired stomatal apertures and hypersensitivity to drought stress. Transcriptomic and chromatin immunoprecipitation analysis revealed that CDK8 positively regulates the transcription of several ABA-responsive genes, probably through promoting the recruitment of RNA polymerase II to their promoters. We discovered that both CDK8 and SnRK2.6 interact physically with an ERF/AP2 transcription factor RAP2.6, which can directly bind to the promoters of RD29A and COLD-REGULATED 15A (COR15A) with GCC or DRE elements, thereby promoting their expression. Importantly, we also showed that CDK8 is essential for the ABA-induced expression of RAP2.6 and RAP2.6-mediated upregulation of ABA-responsive genes, indicating that CDK8 could link the SnRK2.6-mediated ABA signaling to RNA polymerase II to promote immediate transcriptional response to ABA and drought signals. Overall, our data provide new insights into the roles of CDK8 in modulating ABA signaling and drought responses
Beschreibung:Date Completed 26.04.2021
Date Revised 04.12.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.16787