PuHox52-mediated hierarchical multilayered gene regulatory network promotes adventitious root formation in Populus ussuriensis

PuHox52-mediated hierarchical multilayered gene regulatory network promotes adventitious root formation in Populus ussuriensis

© 2020 The Authors. New Phytologist © 2020 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 228(2020), 4 vom: 26. Nov., Seite 1369-1385
1. Verfasser: Wei, Ming (VerfasserIn)
Weitere Verfasser: Liu, Quangang, Wang, Zhanchao, Yang, Jingli, Li, Wenlong, Chen, Yingxi, Lu, Han, Nie, Jinfu, Liu, Baoguang, Lv, Kaiwen, Mao, Xuliang, Chen, Su, Sanders, Jennifer, Wei, Hairong, Li, Chenghao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Populus ussuriensis PuHox52 adventitious root cutting histone acetylation multilayered hierarchical gene regulatory network Indoleacetic Acids
Beschreibung
Zusammenfassung:© 2020 The Authors. New Phytologist © 2020 New Phytologist Foundation.
Adventitious root (AR) formation is critically important in vegetative propagation through cuttings in some plants, especially woody species. However, the underlying molecular mechanisms remain elusive. Here, we report the identification of a poplar homeobox gene, PuHox52, which was induced rapidly (within 15 min) at the basal ends of stems upon cutting and played a key regulatory role in adventitious rooting. We demonstrated that overexpression of PuHox52 significantly increased the number of ARs while suppression of PuHox52 had the opposite effect. A multilayered hierarchical gene regulatory network (ML-hGRN) mediated by PuHox52 was reverse-engineered and demonstrated to govern AR formation. PuHox52 regulated AR formation through upregulation of nine hub regulators, including a jasmonate signaling pathway gene, PuMYC2, and an auxin signaling pathway gene, PuAGL12. We also identified coherent type 4 feed-forward loops within this ML-hGRN; PuHox52 repressed PuHDA9, which encodes a histone deacetylase, and led to an increase in acetylation and presumably expression of three hub regulators, PuWRKY51, PuLBD21 and PuIAA7. Our results indicate that the ML-hGRN mediated by PuHox52 governs AR formation at the basal ends of stem cuttings from poplar trees
Beschreibung:Date Completed 14.05.2021
Date Revised 14.05.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.16778