Accumulation of the RNA polymerase subunit RpoB depends on RNA editing by OsPPR16 and affects chloroplast development during early leaf development in rice

Accumulation of the RNA polymerase subunit RpoB depends on RNA editing by OsPPR16 and affects chloroplast development during early leaf development in rice

©2020 The Authors. New Phytologist ©2020 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 228(2020), 4 vom: 09. Nov., Seite 1401-1416
1. Verfasser: Huang, Weifeng (VerfasserIn)
Weitere Verfasser: Zhang, Yang, Shen, Liqiang, Fang, Qian, Liu, Qun, Gong, Chenbo, Zhang, Chen, Zhou, Yong, Mao, Cui, Zhu, Yongli, Zhang, Jinghong, Chen, Hongping, Zhang, Yu, Lin, Yongjun, Bock, Ralph, Zhou, Fei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Chl biosynthesis RNA editing RNA polymerase chloroplast development plastid transcription Arabidopsis Proteins DNA-Directed RNA Polymerases EC 2.7.7.6
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520 |a Plastid-encoded genes are coordinately transcribed by the nucleus-encoded RNA polymerase (NEP) and the plastid-encoded RNA polymerase (PEP). Resulting primary transcripts are frequently subject to RNA editing by cytidine-to-uridine conversions at specific sites. The physiological role of many editing events is largely unknown. Here, we have used the CRISPR/Cas9 technique in rice to knock out a member of the PLS-DYW subfamily of pentatricopeptide repeat (PPR) proteins. We found that OsPPR16 is responsible for a single editing event at position 545 in the chloroplast rpoB messenger RNA (mRNA), resulting in an amino acid change from serine to leucine in the β-subunit of the PEP. In striking contrast to loss-of-function mutations of the putative orthologue in Arabidopsis, which were reported to have no visible phenotype, knockout of OsPPR16 leads to impaired accumulation of RpoB, reduced expression of PEP-dependent genes, and a pale phenotype during early plant development. Thus, by editing the rpoB mRNA, OsPPR16 is required for faithful plastid transcription, which in turn is required for Chl synthesis and efficient chloroplast development. Our results provide new insights into the interconnection of the finely tuned regulatory mechanisms that operate at the transcriptional and post-transcriptional levels of plastid gene expression 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Chl biosynthesis 
650 4 |a RNA editing 
650 4 |a RNA polymerase 
650 4 |a chloroplast development 
650 4 |a plastid 
650 4 |a transcription 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a DNA-Directed RNA Polymerases  |2 NLM 
650 7 |a EC 2.7.7.6  |2 NLM 
700 1 |a Zhang, Yang  |e verfasserin  |4 aut 
700 1 |a Shen, Liqiang  |e verfasserin  |4 aut 
700 1 |a Fang, Qian  |e verfasserin  |4 aut 
700 1 |a Liu, Qun  |e verfasserin  |4 aut 
700 1 |a Gong, Chenbo  |e verfasserin  |4 aut 
700 1 |a Zhang, Chen  |e verfasserin  |4 aut 
700 1 |a Zhou, Yong  |e verfasserin  |4 aut 
700 1 |a Mao, Cui  |e verfasserin  |4 aut 
700 1 |a Zhu, Yongli  |e verfasserin  |4 aut 
700 1 |a Zhang, Jinghong  |e verfasserin  |4 aut 
700 1 |a Chen, Hongping  |e verfasserin  |4 aut 
700 1 |a Zhang, Yu  |e verfasserin  |4 aut 
700 1 |a Lin, Yongjun  |e verfasserin  |4 aut 
700 1 |a Bock, Ralph  |e verfasserin  |4 aut 
700 1 |a Zhou, Fei  |e verfasserin  |4 aut 
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773 1 8 |g volume:228  |g year:2020  |g number:4  |g day:09  |g month:11  |g pages:1401-1416 
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