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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13199
|2 doi
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|a eng
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|a Avendaño-Herrera, Ruben
|e verfasserin
|4 aut
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| 245 |
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|a Serological diversity in Flavobacterium psychrophilum
|b A critical update using isolates retrieved from Chilean salmon farms
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|c 2020
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| 336 |
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|a Text
|b txt
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|a ƒaComputermedien
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|a Date Completed 29.01.2021
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|a Date Revised 29.01.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 John Wiley & Sons Ltd.
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|a Chile is currently the second largest producer of farmed salmon worldwide, but Flavobacterium psychrophilum, as one of the most detrimental pathogens, is responsible for major losses during the freshwater culturing step in salmonid fish farms. An antigenic study conducted 10 years ago reported four serological groups using 20 F. psychrophilum Chilean strains. To reduce disease outbreaks and to develop vaccine candidates, antigenic knowledge needs to be regularly updated using a significant number of additional recent F. psychrophilum isolates. The present study aimed at investigating the serological diversity of 118 F. psychrophilum isolates collected between 2006 and 2018 from farmed Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch). The current study supports an expansion of the known antigenic groups in Chile from 4 to 14. However, the use of the slide-agglutination technique for serotyping is costly, is labour-intensive and requires significant technical expertise. Addressing these points, the mPCR-based procedure was a very useful tool for serotyping the collected Chilean F. psychrophilum isolates. This technique revealed the presence of diverse mPCR serotypes (i.e. types 0, 1, 2 and 4). Therefore, mPCR should be employed to select the bacterial strain(s) for vaccine development and to conduct follow-up, selective breeding or epidemiological surveillance in Chilean fish farms. Given the presented findings, changes to Chilean fish-farming practices are vital for ensuring the continued productivity and well-being of farmed salmonids
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|a Journal Article
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|a Flavobacterium psychrophilum
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| 650 |
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|a Chilean salmon farms
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| 650 |
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|a serological diversity
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| 650 |
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|a serotype PCR
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1 |
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|a Tapia-Cammas, Diana
|e verfasserin
|4 aut
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1 |
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|a Duchaud, Eric
|e verfasserin
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| 700 |
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|a Irgang, Rute
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 43(2020), 8 vom: 21. Aug., Seite 877-888
|w (DE-627)NLM098166034
|x 1365-2761
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|g volume:43
|g year:2020
|g number:8
|g day:21
|g month:08
|g pages:877-888
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|u http://dx.doi.org/10.1111/jfd.13199
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