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20231225142107.0 |
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cr uuu---uuuuu |
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231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/jfd.13201
|2 doi
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| 028 |
5 |
2 |
|a pubmed24n1037.xml
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|a (DE-627)NLM311363962
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| 035 |
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|a (NLM)32557714
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| 040 |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Bruce, Timothy J
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Cross-protection of a live-attenuated Flavobacterium psychrophilum immersion vaccine against novel Flavobacterium spp. and Chryseobacterium spp. strains
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| 264 |
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1 |
|c 2020
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 29.01.2021
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| 500 |
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|a Date Revised 29.01.2021
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| 500 |
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|a published: Print-Electronic
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a © 2020 John Wiley & Sons Ltd.
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| 520 |
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|a For salmonid producers, a common threat is Flavobacterium psychrophilum. Recent advancements in bacterial coldwater disease (BCWD) management include the development of a live-attenuated immersion vaccine that cross-protects against an array of F. psychrophilum strains. Emerging family Flavobacteriaceae cases associated with clinical disease have been increasing, including pathogenic isolates of Flavobacterium spp. and Chryseobacterium spp. The cross-protective ability of a live-attenuated F. psychrophilum vaccine was determined against three virulent Flavobacteriaceae isolates. Juvenile rainbow trout were vaccinated, developed high F. psychrophilum-specific antibody titres and were challenged with Chryseobacterium spp. isolates (S25 and T28), a Flavobacterium sp. (S21) isolate, a mixed combination of S21:S25:T28, and a standard virulent F. psychrophilum CSF259-93 strain. Results demonstrated strong protection in the CSF259-93 vaccinated group (relative per cent survival (RPS)=94.44%) when compared to the relevant CSF259-93 controls (p < .001). Protection was also observed for vaccinated fish challenged with the S21:S25:T28 mix (RPS = 85.18%; p < .001). However, protection was not observed with the S21, S25 or T28 isolates alone. Analysis of whole-cell lysates revealed differences in protein banding by SDS-PAGE, but conserved antigenic regions by Western blot in S25 and T28. Results demonstrate that this live-attenuated vaccine provided protection against mixed flavobacterial infection and suggest further benefits against flavobacteriosis
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Chryseobacterium spp.
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| 650 |
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4 |
|a Flavobacterium spp.
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| 650 |
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4 |
|a attenuated vaccine
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| 650 |
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4 |
|a cross-protection
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| 650 |
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7 |
|a Antibodies, Bacterial
|2 NLM
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| 650 |
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7 |
|a Bacterial Vaccines
|2 NLM
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| 650 |
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7 |
|a Vaccines, Attenuated
|2 NLM
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| 700 |
1 |
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|a Ma, Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Knupp, Christopher
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Loch, Thomas P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Faisal, Mohamed
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cain, Kenneth D
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 43(2020), 8 vom: 15. Aug., Seite 915-928
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
|
| 773 |
1 |
8 |
|g volume:43
|g year:2020
|g number:8
|g day:15
|g month:08
|g pages:915-928
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jfd.13201
|3 Volltext
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 43
|j 2020
|e 8
|b 15
|c 08
|h 915-928
|