|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM311329284 |
| 003 |
DE-627 |
| 005 |
20250227110243.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2020.05.029
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1037.xml
|
| 035 |
|
|
|a (DE-627)NLM311329284
|
| 035 |
|
|
|a (NLM)32554175
|
| 035 |
|
|
|a (PII)S0981-9428(20)30261-8
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Barsain, Bharati Lalhal
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a PkGPPS.SSU interacts with two PkGGPPS to form heteromeric GPPS in Picrorhiza kurrooa
|b Molecular insights into the picroside biosynthetic pathway
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 10.12.2020
|
| 500 |
|
|
|a Date Revised 14.12.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2020 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a Geranyl geranyl pyrophosphate synthase (GGPPS) is known to form an integral subunit of the heteromeric GPPS (geranyl pyrophosphate synthase) complex and catalyzes the biosynthesis of monoterpene in plants. Picrorhiza kurrooa Royle ex Benth., a medicinally important high altitude plant is known for picroside biomolecules, the monoterpenoids. However, the significance of heteromeric GPPS in P. kurrooa still remains obscure. Here, transient silencing of PkGGPPS was observed to reduce picroside-I (P-I) content by more than 60% as well as picroside-II (P-II) by more than 75%. Thus, PkGGPPS was found to be involved in the biosynthesis of P-I and P-II besides other terpenoids. To unravel the mechanism, small subunit of GPPS (PkGPPS.SSU) was identified from P. kurrooa. Protein-protein interaction studies in yeast as well as bimolecular fluorescence complementation (BiFC) in planta have indicated that large subunit of GPPS PkGPPS.LSUs (PkGGPPS1 and PkGGPPS2) and PkGPPS.SSU form a heteromeric GPPS. Presence of similar conserved domains such as light responsive motifs, low temperature responsive elements (LTRE), dehydration responsive elements (DREs), W Box and MeJA responsive elements in the promoters of PkGPPS.LSU and PkGPPS.SSU documented their involvement in picroside biosynthesis. Further, the tissue specific transcript expression analysis vis-à-vis epigenetic regulation (DNA methylation) of promoters as well as coding regions of PkGPPS.LSU and PkGPPS.SSU has strongly suggested their role in picroside biosynthesis. Taken together, the newly identified PkGPPS.SSU formed the heteromeric GPPS by interacting with PkGPPS.LSUs to synthesize P-I and P-II in P. kurrooa
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a DNA methylation
|
| 650 |
|
4 |
|a GGPPS
|
| 650 |
|
4 |
|a Geranyl pyrophosphate synthase small subunit
|
| 650 |
|
4 |
|a Heteromeric GPPS
|
| 650 |
|
4 |
|a Picroside
|
| 650 |
|
4 |
|a Promoter
|
| 650 |
|
7 |
|a Cinnamates
|2 NLM
|
| 650 |
|
7 |
|a Iridoid Glucosides
|2 NLM
|
| 650 |
|
7 |
|a Monoterpenes
|2 NLM
|
| 650 |
|
7 |
|a picroside I
|2 NLM
|
| 650 |
|
7 |
|a 27409-30-9
|2 NLM
|
| 650 |
|
7 |
|a picroside II
|2 NLM
|
| 650 |
|
7 |
|a 39012-20-9
|2 NLM
|
| 650 |
|
7 |
|a Dimethylallyltranstransferase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.5.1.1
|2 NLM
|
| 700 |
1 |
|
|a Purohit, Anjali
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kumar, Ajay
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Joshi, Robin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hallan, Vipin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yadav, Sudesh Kumar
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 154(2020) vom: 01. Sept., Seite 115-128
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
|
| 773 |
1 |
8 |
|g volume:154
|g year:2020
|g day:01
|g month:09
|g pages:115-128
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2020.05.029
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 154
|j 2020
|b 01
|c 09
|h 115-128
|