Target sequence heterogeneity causes the 'hook effect' in fluorescent dye-based quantitative PCR
The 'hook effect' describes a phenomenon in quantitative PCR (qPCR) amplification curves where fluorescence values decrease following an initial amplification phase. We propose that in intercalating dye-based qPCR, the 'hook effect' is due to the amplification of heterogeneous bu...
| Veröffentlicht in: | BioTechniques. - 1988. - 69(2020), 2 vom: 07. Aug., Seite 80-83 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Letter Research Support, Non-U.S. Gov't hook effect optimization quantitation quantitation artifact quantitative PCR Fluorescent Dyes Intercalating Agents DNA |
| Online verfügbar |
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