|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM310600502 |
| 003 |
DE-627 |
| 005 |
20231225140438.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/nph.16716
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1035.xml
|
| 035 |
|
|
|a (DE-627)NLM310600502
|
| 035 |
|
|
|a (NLM)32479643
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Li, Fangfang
|e verfasserin
|4 aut
|
| 245 |
1 |
2 |
|a A plant RNA virus activates selective autophagy in a UPR-dependent manner to promote virus infection
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 14.05.2021
|
| 500 |
|
|
|a Date Revised 14.05.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2020 Her Majesty the Queen in Right of Canada New Phytologist © 2020 New Phytologist Trust.
|
| 520 |
|
|
|a Autophagy is an evolutionarily conserved pathway in eukaryotes that delivers unwanted cytoplasmic materials to the lysosome/vacuole for degradation/recycling. Stimulated autophagy emerges as an integral part of plant immunity against intracellular pathogens. In this study, we used turnip mosaic virus (TuMV) as a model to investigate the involvement of autophagy in plant RNA virus infection. The small integral membrane protein 6K2 of TuMV, known as a marker of the virus replication site and an elicitor of the unfolded protein response (UPR), upregulates the selective autophagy receptor gene NBR1 in a UPR-dependent manner. NBR1 interacts with TuMV NIb, the RNA-dependent RNA polymerase of the virus replication complex (VRC), and the autophagy cargo receptor/adaptor protein ATG8f. The NIb/NBR1/ATG8f interaction complexes colocalise with the 6K2-stained VRC. Overexpression of NBR1 or ATG8f enhances TuMV replication, and deficiency of NBR1 or ATG8f inhibits virus infection. In addition, ATG8f interacts with the tonoplast-specific protein TIP1 and the NBR1/ATG8f-containing VRC is enclosed by the TIP1-labelled tonoplast. In TuMV-infected cells, numerous membrane-bound viral particles are evident in the vacuole. Altogether these results suggest that TuMV activates and manipulates UPR-dependent NBR1-ATG8f autophagy to target the VRC to the tonoplast to promote viral replication and virion accumulation
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a RNA-dependent RNA polymerase (RdRp)
|
| 650 |
|
4 |
|a autophagy
|
| 650 |
|
4 |
|a potyvirus
|
| 650 |
|
4 |
|a tonoplast
|
| 650 |
|
4 |
|a turnip mosaic virus (TuMV)
|
| 650 |
|
4 |
|a unfolded protein response (UPR)
|
| 650 |
|
4 |
|a vacuole
|
| 650 |
|
4 |
|a virus replication complex (VRC)
|
| 650 |
|
7 |
|a Arabidopsis Proteins
|2 NLM
|
| 650 |
|
7 |
|a Carrier Proteins
|2 NLM
|
| 650 |
|
7 |
|a NBR1 protein, Arabidopsis
|2 NLM
|
| 650 |
|
7 |
|a RNA, Plant
|2 NLM
|
| 650 |
|
7 |
|a Viral Proteins
|2 NLM
|
| 700 |
1 |
|
|a Zhang, Changwei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Tang, Ziwei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Lingrui
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dai, Zhaoji
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lyu, Shanwu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Yinzi
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hou, Xilin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bernards, Mark
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Aiming
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 228(2020), 2 vom: 02. Okt., Seite 622-639
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
|
| 773 |
1 |
8 |
|g volume:228
|g year:2020
|g number:2
|g day:02
|g month:10
|g pages:622-639
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/nph.16716
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 228
|j 2020
|e 2
|b 02
|c 10
|h 622-639
|