Characterization of O-methyltransferases involved in the biosynthesis of tetrandrine in Stephania tetrandra

Characterization of O-methyltransferases involved in the biosynthesis of tetrandrine in Stephania tetrandra

Copyright © 2020 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 250(2020) vom: 26. Juli, Seite 153181
1. Verfasser: Li, Qishuang (VerfasserIn)
Weitere Verfasser: Bu, Junling, Ma, Ying, Yang, Jian, Hu, Zhimin, Lai, Changjiangsheng, Xu, Yanqin, Tang, Jinfu, Cui, Guanghong, Wang, Yanan, Zhao, Yujun, Jin, Baolong, Shen, Ye, Guo, Juan, Huang, Luqi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article (S)-norcoclaurine-6-O-methyltransferase (6OMT) Benzylisoquinoline alkaloid (BIA) biosynthesis Functional characterization Tetrandrine Antiviral Agents Benzylisoquinolines Plant Proteins tetrandrine 29EX23D5AJ mehr... Methyltransferases EC 2.1.1.- S-adenosyl-L-methionine-norcoclaurine 6-O-methyltransferase
Beschreibung
Zusammenfassung:Copyright © 2020 Elsevier GmbH. All rights reserved.
Tetrandrine is the most effective small molecule that has been found to inhibit the Ebola virus. It is a typical bisbenzylisoquinoline alkaloid and is the main active ingredient in Stephania tetrandra. Metabolic engineering and synthetic biology are potential methods for efficient and rapid acquisition of tetrandrine. S-adenosyl-L-methionine: (S)-norcoclaurine-6-O-methyltransferase (6OMT) is a rate-limiting step involved in the biosynthesis of tetrandrine. In this study, we identify S-adenosyl-L-methionine: (S)-norcoclaurine-6-O-methyltransferase from S. tetrandra, which catalyzes the conversion of (S)-norcoclaurine to (S)-coclaurine. Four 6OMT-like genes were cloned from S. tetrandra. An in vitro enzyme assay showed that St6OMT1 could catalyze the conversion of (S)-norcoclaurine to produce (S)-coclaurine. St6OMT2 can catalyze the production of very few (S)-coclaurine molecules, accompanied by more by-products with m/z 300, compared to St6OMT1. The newly discovered 6OMTs will provide an optional genetic component for benzylisoquinoline alkaloid (BIA) synthetic biology research. This work will lay the foundation for the analysis of the biosynthetic pathway of tetrandrine in S. tetrandra
Beschreibung:Date Completed 13.10.2020
Date Revised 13.10.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1618-1328
DOI:10.1016/j.jplph.2020.153181