Identification of novel OsCML16 target proteins and differential expression analysis under abiotic stresses in rice

Copyright © 2020. Published by Elsevier GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 249(2020) vom: 22. Juni, Seite 153165
1. Verfasser: Yang, Jun (VerfasserIn)
Weitere Verfasser: Liu, Shuang, Ji, Lingxiao, Tang, Xianying, Zhu, Yongsheng, Xie, Guosheng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Abiotic stress Calmodulin-binding domain Calmodulin-like protein Expression Interaction Rice Plant Proteins
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520 |a Calmodulin-like proteins (CMLs) have been shown to play key regulatory roles in calcium signaling in plants. However, few bona-fide CMLs binding proteins have been characterized in rice, a monocot model plant. Here, through large-scale screening of a yeast-two hybrid (Y2H) cDNA library with OsCML16 as a bait, six new putative interacting partners of OsCML16 were discovered and confirmed by both pairwise Y2H and bimolecular fluorescence complementation (BiFC) assays. Interestingly, the in vitro peptide-binding assays manifested that OsERD2 could bind both OsCaM1 and OsCML16 whereas other five target proteins could specifically bind OsCML16 but not OsCaM1. Furthermore, Ca2+ and TFP, a calmodulin (CaM) antagonist, were involved in the ABA-induced transcription of OsCML16 and its target genes, and they were also obviously induced by cold, drought, and salt stresses. Taken together, our new findings have provided the basis for the novel signaling pathways of OsCML16 in the abiotic stress response in rice 
650 4 |a Journal Article 
650 4 |a Abiotic stress 
650 4 |a Calmodulin-binding domain 
650 4 |a Calmodulin-like protein 
650 4 |a Expression 
650 4 |a Interaction 
650 4 |a Rice 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Liu, Shuang  |e verfasserin  |4 aut 
700 1 |a Ji, Lingxiao  |e verfasserin  |4 aut 
700 1 |a Tang, Xianying  |e verfasserin  |4 aut 
700 1 |a Zhu, Yongsheng  |e verfasserin  |4 aut 
700 1 |a Xie, Guosheng  |e verfasserin  |4 aut 
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