Flavonoids are indispensable for complete male fertility in rice

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 71(2020), 16 vom: 06. Aug., Seite 4715-4728
1. Verfasser:	Wang, Lanxiang (VerfasserIn)
Weitere Verfasser:	Lam, Pui Ying, Lui, Andy C W, Zhu, Fu-Yuan, Chen, Mo-Xian, Liu, Hongjia, Zhang, Jianhua, Lo, Clive
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Oryza sativa Chalcone synthase flavanones flavone C-glycosides flavones flavonols male fertility rice mehr... Flavonoids Flavonols

Beschreibung
Zusammenfassung:	© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Flavonoids are essential for male fertility in some but not all plant species. In rice (Oryza sativa), the chalcone synthase mutant oschs1 produces flavonoid-depleted pollen and is male sterile. The mutant pollen grains are viable with normal structure, but they display reduced germination rate and pollen-tube length. Analysis of oschs1/+ heterozygous lines shows that pollen flavonoid deposition is a paternal effect and fertility is independent of the haploid genotypes (OsCHS1 or oschs1). To understand which classes of flavonoids are involved in male fertility, we conducted detailed analysis of rice mutants for branch-point enzymes of the downstream flavonoid pathways, including flavanone 3-hydroxylase (OsF3H; flavonol pathway entry enzyme), flavone synthase II (CYP93G1; flavone pathway entry enzyme), and flavanone 2-hydroxylase (CYP93G2; flavone C-glycoside pathway entry enzyme). Rice osf3h and cyp93g1 cyp93g2 CRISPR/Cas9 mutants, and cyp93g1 and cyp93g2 T-DNA insertion mutants showed altered flavonoid profiles in anthers, but only the osf3h and cyp93g1 cyp93g2 mutants displayed reduction in seed yield. Our findings indicate that flavonoids are essential for complete male fertility in rice and a combination of different classes (flavanones, flavonols, flavones, and flavone C-glycosides) appears to be important, as opposed to the essential role played primarily by flavonols that has been previously reported in several plant species
Beschreibung:	Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/eraa204