Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria

Anaerobic ammonium-oxidizing (anammox) bacteria play a key role in the global nitrogen cycle and in nitrogenous wastewater treatment. The anammox bacteria ultrastructure is unique and distinctly different from that of other prokaryotic cells. The morphological structure of an organism is related to...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 27(2020), Pt 3 vom: 01. Mai, Seite 753-761
1. Verfasser: Peng, Meng Wen (VerfasserIn)
Weitere Verfasser: Guan, Yong, Liu, Jian Hong, Chen, Liang, Wang, Han, Xie, Zheng Zhe, Li, Hai Yan, Chen, You Peng, Liu, Peng, Yan, Peng, Guo, Jin Song, Liu, Gang, Shen, Yu, Fang, Fang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article anammox iron synchrotron three-dimensional nondestructive-imaging Ammonium Compounds
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520 |a Anaerobic ammonium-oxidizing (anammox) bacteria play a key role in the global nitrogen cycle and in nitrogenous wastewater treatment. The anammox bacteria ultrastructure is unique and distinctly different from that of other prokaryotic cells. The morphological structure of an organism is related to its function; however, research on the ultrastructure of intact anammox bacteria is lacking. In this study, in situ three-dimensional nondestructive ultrastructure imaging of a whole anammox cell was performed using synchrotron soft X-ray tomography (SXT) and the total variation-based simultaneous algebraic reconstruction technique (TV-SART). Statistical and quantitative analyses of the intact anammox bacteria were performed. High soft X-ray absorption composition inside anammoxosome was detected and verified to be relevant to iron-binding protein. On this basis, the shape adaptation of the anammox bacteria response to iron was explored 
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700 1 |a Guan, Yong  |e verfasserin  |4 aut 
700 1 |a Liu, Jian Hong  |e verfasserin  |4 aut 
700 1 |a Chen, Liang  |e verfasserin  |4 aut 
700 1 |a Wang, Han  |e verfasserin  |4 aut 
700 1 |a Xie, Zheng Zhe  |e verfasserin  |4 aut 
700 1 |a Li, Hai Yan  |e verfasserin  |4 aut 
700 1 |a Chen, You Peng  |e verfasserin  |4 aut 
700 1 |a Liu, Peng  |e verfasserin  |4 aut 
700 1 |a Yan, Peng  |e verfasserin  |4 aut 
700 1 |a Guo, Jin Song  |e verfasserin  |4 aut 
700 1 |a Liu, Gang  |e verfasserin  |4 aut 
700 1 |a Shen, Yu  |e verfasserin  |4 aut 
700 1 |a Fang, Fang  |e verfasserin  |4 aut 
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773 1 8 |g volume:27  |g year:2020  |g number:Pt 3  |g day:01  |g month:05  |g pages:753-761 
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