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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202000036
|2 doi
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|a pubmed24n1032.xml
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|a (NLM)32378244
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a Chen, Yaping
|e verfasserin
|4 aut
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|a Silicon-Nanotube-Mediated Intracellular Delivery Enables Ex Vivo Gene Editing
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 12.04.2021
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|a Date Revised 12.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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|a Engineered nano-bio cellular interfaces driven by vertical nanostructured materials are set to spur transformative progress in modulating cellular processes and interrogations. In particular, the intracellular delivery-a core concept in fundamental and translational biomedical research-holds great promise for developing novel cell therapies based on gene modification. This study demonstrates the development of a mechanotransfection platform comprising vertically aligned silicon nanotube (VA-SiNT) arrays for ex vivo gene editing. The internal hollow structure of SiNTs allows effective loading of various biomolecule cargoes; and SiNTs mediate delivery of those cargoes into GPE86 mouse embryonic fibroblasts without compromising their viability. Focused ion beam scanning electron microscopy (FIB-SEM) and confocal microscopy results demonstrate localized membrane invaginations and accumulation of caveolin-1 at the cell-NT interface, suggesting the presence of endocytic pits. Small-molecule inhibition of endocytosis suggests that active endocytic process plays a role in the intracellular delivery of cargo from SiNTs. SiNT-mediated siRNA intracellular delivery shows the capacity to reduce expression levels of F-actin binding protein (Triobp) and alter the cellular morphology of GPE86. Finally, the successful delivery of Cas9 ribonucleoprotein (RNP) to specifically target mouse Hprt gene is achieved. This NT-enhanced molecular delivery platform has strong potential to support gene editing technologies
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|a Journal Article
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|a Cas9 RNP
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|a gene editing
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|a intracellular delivery
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|a siRNA knockdown
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|a silicon nanotubes
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|a Caveolin 1
|2 NLM
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|a RNA, Small Interfering
|2 NLM
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|a Silicon
|2 NLM
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|a Z4152N8IUI
|2 NLM
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| 700 |
1 |
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|a Aslanoglou, Stella
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Murayama, Takahide
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gervinskas, Gediminas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fitzgerald, Laura I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sriram, Sharath
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tian, Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Johnston, Angus P R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Morikawa, Yasuhiro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Suu, Koukou
|e verfasserin
|4 aut
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|a Elnathan, Roey
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Voelcker, Nicolas H
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 32(2020), 24 vom: 30. Juni, Seite e2000036
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
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|g volume:32
|g year:2020
|g number:24
|g day:30
|g month:06
|g pages:e2000036
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|u http://dx.doi.org/10.1002/adma.202000036
|3 Volltext
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