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231225s2020 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2020-0025
|2 doi
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|a pubmed24n1031.xml
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|a (NLM)32372656
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a You, Jae Bem
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Nanoadhesive layer to prevent protein absorption in a poly(dimethylsiloxane) microfluidic device
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| 264 |
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|c 2020
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 14.08.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Poly(dimethylsiloxane) (PDMS) is widely used as a microfluidics platform material; however, it absorbs various molecules, perturbing specific chemical concentrations in microfluidic channels. We present a simple solution to prevent adsorption into a PDMS microfluidic device. We used a vapor-phase-deposited nanoadhesive layer to seal PDMS microfluidic channels. Absorption of fluorescent molecules into PDMS was efficiently prevented in the nanolayer-treated PDMS device. Importantly, when cultured in a nanolayer-treated PDMS device, yeast cells exhibited the expected concentration-dependent response to a mating pheromone, including mating-specific morphological and gene expression changes, while yeast cultured in an untreated PDMS device did not properly respond to the pheromone. Our method greatly expands microfluidic applications that require precise control of molecule concentrations
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a PDMS microfluidics
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4 |
|a device bonding
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| 650 |
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4 |
|a nanoadhesive layer
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| 650 |
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4 |
|a protein absorption
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| 650 |
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4 |
|a yeast mating
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| 700 |
1 |
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|a Lee, Byungjin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Choi, Yunho
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Chang-Soo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Peter, Matthias
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Im, Sung Gap
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Sung Sik
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 69(2020), 1 vom: 03. Juli, Seite 404-409
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:69
|g year:2020
|g number:1
|g day:03
|g month:07
|g pages:404-409
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| 856 |
4 |
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|u http://dx.doi.org/10.2144/btn-2020-0025
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|d 69
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