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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1270/jsbbs.19082
|2 doi
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|a pubmed24n1031.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Sakamoto, Shingo
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Efficient transient gene expression system using buckwheat hypocotyl protoplasts for large-scale experiments
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 28.09.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Copyright © 2020 by JAPANESE SOCIETY OF BREEDING.
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|a Buckwheat (Fagopyrum esculentum) is cultivated worldwide and its flour is used in a variety of food products. Although functional analyses of genes in buckwheat are highly desired, reliable methods to do it have yet to be developed. In this study we established a simple and efficient transient gene expression system using buckwheat protoplasts isolated from young hypocotyls using 96-well plates as a high-throughput platform. The transformation efficiency was comparable with that of similar systems, such as Arabidopsis mesophyll protoplasts. Stable results were obtained in a typical example of the experiment to examine transcription factor activity. This system shows potential for the large-scale analysis of gene function using protoplast isolated from fewer and younger plants than the conventional system and may provide novel information for efficient buckwheat breeding
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|a Journal Article
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|a 96-well plate
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| 650 |
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4 |
|a buckwheat
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| 650 |
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4 |
|a effector–reporter analysis
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| 650 |
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4 |
|a hypocotyl protoplast
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| 650 |
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4 |
|a transient gene expression
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| 700 |
1 |
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|a Matsui, Katsuhiro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Oshima, Yoshimi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mitsuda, Nobutaka
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Breeding science
|d 1998
|g 70(2020), 1 vom: 21. März, Seite 128-134
|w (DE-627)NLM098238299
|x 1344-7610
|7 nnns
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| 773 |
1 |
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|g volume:70
|g year:2020
|g number:1
|g day:21
|g month:03
|g pages:128-134
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| 856 |
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|u http://dx.doi.org/10.1270/jsbbs.19082
|3 Volltext
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