Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 71(2020), 12 vom: 22. Juni, Seite 3560-3574
1. Verfasser: Gao, Ying (VerfasserIn)
Weitere Verfasser: Wei, Wei, Fan, Zhongqi, Zhao, Xiaodan, Zhang, Yiping, Jing, Yuan, Zhu, Benzhong, Zhu, Hongliang, Shan, Wei, Chen, Jianye, Grierson, Donald, Luo, Yunbo, Jemrić, Tomislav, Jiang, Cai-Zhong, Fu, Da-Qi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't NAC-NOR nor mutant CRISPR/Cas9 gene function tomato fruit ripening transcription factor Ethylenes Plant Proteins Transcription Factors
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245 1 0 |a Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening 
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500 |a ErratumIn: J Exp Bot. 2020 Jun 22;71(12):3759. - PMID 32497178 
500 |a Citation Status MEDLINE 
520 |a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. 
520 |a The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be a gain-of-function mutant. Here, we provide more evidence to support this view and answer the open question of whether the NAC-NOR gene is important in fruit ripening. Overexpression of NAC-NOR in the nor mutant did not restore the full ripening phenotype. Further analysis showed that the truncated NOR186 protein is located in the nucleus and binds to but does not activate the promoters of 1-aminocyclopropane-1-carboxylic acid synthase2 (SlACS2), geranylgeranyl diphosphate synthase2 (SlGgpps2), and pectate lyase (SlPL), which are involved in ethylene biosynthesis, carotenoid accumulation, and fruit softening, respectively. The activation of the promoters by the wild-type NOR protein can be inhibited by the mutant NOR186 protein. On the other hand, ethylene synthesis, carotenoid accumulation, and fruit softening were significantly inhibited in CR-NOR (CRISPR/Cas9-edited NAC-NOR) fruit compared with the wild-type, but much less severely affected than in the nor mutant, while they were accelerated in OE-NOR (overexpressed NAC-NOR) fruit. These data further indicated that nor is a gain-of-function mutation and NAC-NOR plays a significant role in ripening of wild-type fruit 
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650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a NAC-NOR 
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650 4 |a CRISPR/Cas9 
650 4 |a gene function 
650 4 |a tomato fruit ripening 
650 4 |a transcription factor 
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700 1 |a Wei, Wei  |e verfasserin  |4 aut 
700 1 |a Fan, Zhongqi  |e verfasserin  |4 aut 
700 1 |a Zhao, Xiaodan  |e verfasserin  |4 aut 
700 1 |a Zhang, Yiping  |e verfasserin  |4 aut 
700 1 |a Jing, Yuan  |e verfasserin  |4 aut 
700 1 |a Zhu, Benzhong  |e verfasserin  |4 aut 
700 1 |a Zhu, Hongliang  |e verfasserin  |4 aut 
700 1 |a Shan, Wei  |e verfasserin  |4 aut 
700 1 |a Chen, Jianye  |e verfasserin  |4 aut 
700 1 |a Grierson, Donald  |e verfasserin  |4 aut 
700 1 |a Luo, Yunbo  |e verfasserin  |4 aut 
700 1 |a Jemrić, Tomislav  |e verfasserin  |4 aut 
700 1 |a Jiang, Cai-Zhong  |e verfasserin  |4 aut 
700 1 |a Fu, Da-Qi  |e verfasserin  |4 aut 
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