Higher PIK3C2B gene expression of H1N1+ specific B-cells is associated with lower H1N1 immunogenicity after trivalent influenza vaccination in HIV infected children

Higher PIK3C2B gene expression of H1N1+ specific B-cells is associated with lower H1N1 immunogenicity after trivalent influenza vaccination in HIV infected children

Copyright © 2020 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 215(2020) vom: 16. Juni, Seite 108440
1. Verfasser: Cotugno, Nicola (VerfasserIn)
Weitere Verfasser: Zicari, Sonia, Morrocchi, Elena, de Armas, Lesley R, Pallikkuth, Suresh, Rinaldi, Stefano, Ruggiero, Alessandra, Manno, Emma Concetta, Zangari, Paola, Chiriaco, Maria, Bernardi, Stefania, Andrews, Sarah F, Cagigi, Alberto, Rossi, Paolo, McDermott, Adrian B, Pahwa, Savita, Palma, Paolo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antigen specific B cells Gene expression H1N1 Influenza vaccination PIK3C2B Perinatally HIV infected children Antibodies, Viral mehr... Influenza Vaccines Class II Phosphatidylinositol 3-Kinases EC 2.7.1.137 PIK3C2B protein, human
Beschreibung
Zusammenfassung:Copyright © 2020 Elsevier Inc. All rights reserved.
Perinatally HIV-infected children (PHIV), despite successful antiretroviral therapy, present suboptimal responses to vaccinations compared to healthy-controls (HC). Here we investigated phenotypic and transcriptional signatures of H1N1-specific B-cells (H1N1-Sp) in PHIV, differentially responding to trivalent-influenza-vaccine (TIV), and HC. Patients were categorized in responders (R) and non-responders (NR) according to hemagglutination-inhibition-assay at baseline and 21 days after TIV. No differences in H1N1-Sp frequencies were found between groups. H1N1-Sp transcriptional analysis revealed a distinct signature between PHIV and HC. NR presented higher PIK3C2B and NOD2 expression compared to R, confirmed by downregulation of PIK3C2B in resting-memory of R after H1N1 in-vitro stimulation. In conclusion this study confirms that qualitative rather than quantitative analyses are needed to characterize immune responses in PHIV. These results further suggest that higher PIK3C2B in H1N1-Sp of NR is associated with lower H1N1 immunogenicity and may be targeted by future modulating strategies to improve TIV responses in PHIV
Beschreibung:Date Completed 29.01.2021
Date Revised 30.12.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2020.108440