Optimization of western blotting for the detection of proteins of different molecular weight

Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended...

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Publié dans:	BioTechniques. - 1993. - 68(2020), 6 vom: 05. Juni, Seite 318-324
Auteur principal:	Heda, Ghanshyam D (Auteur)
Autres auteurs:	Shrestha, Lisa, Thapa, Sagarina, Ghimire, Shreya, Raut, Diptika
Format:	Article en ligne
Langue:	English
Publié:	2020
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. CFTR LAMP1 Rab11a Towbin’s transfer buffer blocking agents methanol concentration optimization plus... western blotting CFTR protein, human Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6 Collodion 9004-70-0