Optimization of western blotting for the detection of proteins of different molecular weight
Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 68(2020), 6 vom: 05. Juni, Seite 318-324
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| 1. Verfasser: |
Heda, Ghanshyam D
(VerfasserIn) |
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| Weitere Verfasser: |
Shrestha, Lisa,
Thapa, Sagarina,
Ghimire, Shreya,
Raut, Diptika |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
CFTR
LAMP1
Rab11a
Towbin’s transfer buffer
blocking agents
methanol concentration
optimization
mehr...
western blotting
CFTR protein, human
Cystic Fibrosis Transmembrane Conductance Regulator
126880-72-6
Collodion
9004-70-0 |
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