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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1107/S1600576720002423
|2 doi
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Lee, Donghyeon
|e verfasserin
|4 aut
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|a Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 24.08.2024
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|a published: Electronic-eCollection
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|a Citation Status PubMed-not-MEDLINE
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|a © Donghyeon Lee et al. 2020.
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|a Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution
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|a Journal Article
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|a HT-MCD
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|a X-ray free-electron lasers
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|a XFELs
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|a high-throughput microcrystal delivery
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|a microcrystal delivery systems
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|a serial femtosecond crystallography
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1 |
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|a Park, Sehan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Keondo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Jangwoo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Park, Gisu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nam, Ki Hyun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baek, Sangwon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chung, Wan Kyun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Jong-Lam
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cho, Yunje
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Park, Jaehyun
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of applied crystallography
|d 1998
|g 53(2020), Pt 2 vom: 01. Apr., Seite 477-485
|w (DE-627)NLM098121561
|x 0021-8898
|7 nnas
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| 773 |
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|g volume:53
|g year:2020
|g number:Pt 2
|g day:01
|g month:04
|g pages:477-485
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|u http://dx.doi.org/10.1107/S1600576720002423
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