NaStEP, an essential protein for self-incompatibility in Nicotiana, performs a dual activity as a proteinase inhibitor and as a voltage-dependent channel blocker

Copyright © 2020 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 151(2020) vom: 06. Juni, Seite 352-361
1. Verfasser: Cruz-Zamora, Y (VerfasserIn)
Weitere Verfasser: Nájera-Torres, E, Noriega-Navarro, R, Torres-Rodríguez, M D, Bernal-Gracida, L A, García-Valdés, J, Juárez-Díaz, J A, Cruz-García, F
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Kunitz-type inhibitor Nicotiana alata Oocytes Pollen rejection Subtilisin Xenopus Membrane Transport Modulators Plant Proteins Protease Inhibitors mehr... Peptide Hydrolases EC 3.4.-
LEADER 01000caa a22002652 4500
001 NLM308575016
003 DE-627
005 20231227130231.0
007 cr uuu---uuuuu
008 231225s2020 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.plaphy.2020.03.052  |2 doi 
028 5 2 |a pubmed24n1224.xml 
035 |a (DE-627)NLM308575016 
035 |a (NLM)32272353 
035 |a (PII)S0981-9428(20)30165-0 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Cruz-Zamora, Y  |e verfasserin  |4 aut 
245 1 0 |a NaStEP, an essential protein for self-incompatibility in Nicotiana, performs a dual activity as a proteinase inhibitor and as a voltage-dependent channel blocker 
264 1 |c 2020 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 19.08.2020 
500 |a Date Revised 13.12.2023 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2020 Elsevier Masson SAS. All rights reserved. 
520 |a The S-specific pollen rejection response in Nicotiana depends on the interaction between S-RNase and a suite of SLF proteins. However, the biochemical pathway requires other essential proteins. One of them is the stigmatic protein NaStEP, which belongs to the Kunitz-type protease inhibitor family. Within the pollen tubes, NaStEP is a positive regulator of HT-B stability, likely inhibiting its degradation and, additionally, interacts with NaSIPP, a mitochondrial phosphate carrier. To gain a deeper understanding of the biochemical role of NaStEP in pollen rejection, we evaluated whether the activity of NaStEP as protease inhibitor is specific to a particular type of protease and whether it has the function of a voltage-dependent channel (VDC) blocker. Our findings indicate that, in vitro, NaStEP inhibits a subtilisin-like protease in an irreversible manner, but not other proteases, such as thermolysin and papain. Furthermore, we found that subtilisin processes the native NaStEP (24 kDa) into two lower molecular weight peptides of 21 and 14 kDa. Moreover, when we incubated NaStEP along with Xenopus leavis oocytes expressing the voltage-dependent potassium channel Kv 1.3, the current was blocked, indicating that NaStEP acts as a VDC blocker. These data allow us to propose NaStEP acts as a key molecule with two functions, one protecting HT-B from degradation by inhibiting a subtilisin-like protease and the second one by forming a complex with a mitochondrial VDC that could destabilize the mitochondria to trigger cell death, which would reinforce S-specific pollen rejection in Nicotiana 
650 4 |a Journal Article 
650 4 |a Kunitz-type inhibitor 
650 4 |a Nicotiana alata 
650 4 |a Oocytes 
650 4 |a Pollen rejection 
650 4 |a Subtilisin 
650 4 |a Xenopus 
650 7 |a Membrane Transport Modulators  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Protease Inhibitors  |2 NLM 
650 7 |a Peptide Hydrolases  |2 NLM 
650 7 |a EC 3.4.-  |2 NLM 
700 1 |a Nájera-Torres, E  |e verfasserin  |4 aut 
700 1 |a Noriega-Navarro, R  |e verfasserin  |4 aut 
700 1 |a Torres-Rodríguez, M D  |e verfasserin  |4 aut 
700 1 |a Bernal-Gracida, L A  |e verfasserin  |4 aut 
700 1 |a García-Valdés, J  |e verfasserin  |4 aut 
700 1 |a Juárez-Díaz, J A  |e verfasserin  |4 aut 
700 1 |a Cruz-García, F  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 151(2020) vom: 06. Juni, Seite 352-361  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:151  |g year:2020  |g day:06  |g month:06  |g pages:352-361 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2020.03.052  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 151  |j 2020  |b 06  |c 06  |h 352-361