Gold Nanocluster Extracellular Vesicle Supraparticles Self-Assembled Nanostructures for Three-Dimensional Uptake Visualization

Gold Nanocluster Extracellular Vesicle Supraparticles : Self-Assembled Nanostructures for Three-Dimensional Uptake Visualization

Extracellular vesicles (EVs) are secreted by the vast majority of cells and are being intensively studied due to their emerging involvement in a variety of cellular communication processes. However, the study of their cellular uptake and fate has been hampered by difficulty in imaging EVs against th...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 36(2020), 14 vom: 14. Apr., Seite 3912-3923
1. Verfasser: Kauscher, Ulrike (VerfasserIn)
Weitere Verfasser: Penders, Jelle, Nagelkerke, Anika, Holme, Margaret N, Nele, Valeria, Massi, Lucia, Gopal, Sahana, Whittaker, Thomas E, Stevens, Molly M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't
Beschreibung
Zusammenfassung:Extracellular vesicles (EVs) are secreted by the vast majority of cells and are being intensively studied due to their emerging involvement in a variety of cellular communication processes. However, the study of their cellular uptake and fate has been hampered by difficulty in imaging EVs against the cellular background. Here, we show that EVs combined with hydrophobic gold nanoclusters (AuNCs) can self-assemble into supraparticles, offering an excellent labeling strategy for high-resolution electron microscopic imaging in vitro. We have tracked and visualized the reuptake of breast cancer cell-derived EV AuNC supraparticles into their parent cells, from early endocytosis to lysosomal degradation, using focused ion beam-scanning electron microscopy (FIB-SEM). The presence of gold within the EVs and lysosomes was confirmed via DF-STEM EDX analysis of lift-out sections. The demonstrated formation of AuNC EV supraparticles will facilitate future applications in EV imaging as well as the EV-assisted cellular delivery of AuNCs
Beschreibung:Date Completed 13.08.2020
Date Revised 10.01.2021
published: Print-Electronic
Citation Status PubMed-not-MEDLINE
ISSN:1520-5827
DOI:10.1021/acs.langmuir.9b03479