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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2020.110459
|2 doi
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|a pubmed25n1027.xml
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|a (DE-627)NLM308197917
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|a (NLM)32234218
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|a (PII)S0168-9452(20)30061-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Liu, Hai-Jing
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 23.11.2020
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|a Date Revised 23.11.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2020 Elsevier B.V. All rights reserved.
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|a Glutathione reductase (GR; EC 1.6.4.2) is a key NADPH-dependent flavo-protein oxidoreductase which can catalyze the oxidized glutathione (GSSG) to reduced glutathione (GSH) to protect plant cells from oxidative damage induced by Reactive oxygen species (ROS) burst. To investigate the biochemical characteristics and functional divergence of Populus GR family, three GR genes (PtGR1.1/1.2/2) were cloned from Populus trichocarpa and their biochemical characteristics were analyzed in this study. All the three genes were expressed in root, stem, leaf and bud, and the expression of PtGR genes were general upregulated under salicylic acid and alamethicin treatment. PtGR1.1 and PtGR1.2 were localized in cytoplasm, while PtGR2 was in chloroplast. The three PtGR proteins showed different enzymatic activities, apparent kinetic characteristic and thermal stability profiles. However, they have similar bivalent metal ions (Cu2+, Cd2+, Zn2+ and Pb2+) sensitivity and optimum pH profiles. Our study sheds light on a comprehensive information of glutathione reductase family in P. trichocarpa, and proved PtGR genes play critical roles when suffering different stresses
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|a Journal Article
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|a Biochemical characteristics
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|a Expression pattern
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|a Glutathione reductase
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|a Populus trichocarpa
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|a Subcellular localization
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|a Plant Proteins
|2 NLM
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|a Reactive Oxygen Species
|2 NLM
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|a Cadmium
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|a 00BH33GNGH
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|a Alamethicin
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|a 27061-78-5
|2 NLM
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|a Lead
|2 NLM
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|a 2P299V784P
|2 NLM
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|a Copper
|2 NLM
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|a 789U1901C5
|2 NLM
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|a Glutathione Reductase
|2 NLM
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|a EC 1.8.1.7
|2 NLM
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|a Zinc
|2 NLM
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|a J41CSQ7QDS
|2 NLM
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|a Salicylic Acid
|2 NLM
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|a O414PZ4LPZ
|2 NLM
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| 700 |
1 |
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|a Wang, Xin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Zhi-Ling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ren, Lin-Ling
|e verfasserin
|4 aut
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| 700 |
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|a Qian, Ting-Ting
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 294(2020) vom: 01. Mai, Seite 110459
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
1 |
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|g volume:294
|g year:2020
|g day:01
|g month:05
|g pages:110459
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| 856 |
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|u http://dx.doi.org/10.1016/j.plantsci.2020.110459
|3 Volltext
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|d 294
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