A novel method for removing polyethyleneimine from biopharmaceutical samples : improving assay sensitivity of residual DNA qPCR
Polyethyleneimine (PEI) is a flocculent that is widely used in the downstream purification of monoclonal antibodies. It is an in-process residual that is carried through the drug purification process and strongly inhibits residual DNA quantitation by real-time quantitative PCR assay. Very high sampl...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 68(2020), 6 vom: 15. Juni, Seite 353-358
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| 1. Verfasser: |
Zhang, Shu-Min
(VerfasserIn) |
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| Weitere Verfasser: |
Roberts, Matthew,
Jones, Marisa,
Zeitler, Jacob,
Kilby, Greg,
Liu, Aston,
White, John R |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
PEI
heparin
host cell DNA
qPCR
sarkosyl
spike recovery
Biological Products
Polyethyleneimine
9002-98-6
mehr...
DNA
9007-49-2 |
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