KNOX protein KNAT1 regulates fruitlet abscission in litchi by repressing ethylene biosynthetic genes

KNOX protein KNAT1 regulates fruitlet abscission in litchi by repressing ethylene biosynthetic genes

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 71(2020), 14 vom: 06. Juli, Seite 4069-4082
1. Verfasser: Zhao, Minglei (VerfasserIn)
Weitere Verfasser: Li, Caiqin, Ma, Xingshuai, Xia, Rui, Chen, Jianye, Liu, Xuncheng, Ying, Peiyuan, Peng, Manjun, Wang, Jun, Shi, Chun-Lin, Li, Jianguo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ACS/ACO KNAT1 ethylene biosynthesis fruitlet abscission litchi (Litchi chinensis Sonn.) transcription Arabidopsis Proteins Ethylenes mehr... Homeodomain Proteins KNAT1 protein, Arabidopsis Plant Proteins
Beschreibung
Zusammenfassung:© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
Abscission is triggered by multiple environmental and developmental cues, including endogenous plant hormones. KNOTTED-LIKE HOMEOBOX (KNOX) transcription factors (TFs) play an important role in controlling abscission in plants. However, the underlying molecular mechanism of KNOX TFs in abscission is largely unknown. Here, we identified LcKNAT1, a KNOTTED-LIKE FROM ARABIDOPSIS THALIANA1 (KNAT1)-like protein from litchi, which regulates abscission by modulating ethylene biosynthesis. LcKNAT1 is expressed in the fruit abscission zone and its expression decreases during fruitlet abscission. Furthermore, the expression of the ethylene biosynthetic genes LcACS1, LcACS7, and LcACO2 increases in the fruit abscission zone, in parallel with the emission of ethylene in fruitlets. In vitro and in vivo assays revealed that LcKNAT1 inhibits the expression of LcACS/ACO genes by directly binding to their promoters. Moreover, ectopic expression of LcKNAT1 represses flower abscission in tomatoes. Transgenic plants expressing LcKNAT1 also showed consistently decreased expression of ACS/ACO genes. Collectively, these results indicate that LcKNAT1 represses abscission via the negative regulation of ethylene biosynthesis
Beschreibung:Date Completed 14.05.2021
Date Revised 14.05.2021
published: Print
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/eraa162