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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.0c00103
|2 doi
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|a pubmed25n1026.xml
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|a (NLM)32212624
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lau, Derrick
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Self-Assembly of Fluorescent HIV Capsid Spheres for Detection of Capsid Binders
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 26.05.2021
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|a Date Revised 26.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The human immunodeficiency virus (HIV) capsid is a cone-shaped capsule formed from the viral capsid protein (CA), which is arranged into a lattice of hexamers and pentamers. The capsid comprises multiple binding interfaces for the recruitment of host proteins and macromolecules used by the virus to establish infection. Here, we coassembled CA proteins engineered for pentamer cross-linking and fluorescence labeling, into spherical particles. The CA spheres, which resemble the pentamer-rich structure of the end caps of the native HIV capsid, were immobilized onto surfaces as biorecognition elements for fluorescence microscopy-based quantification of host protein binding. The capsid-binding host protein cyclophilin A (CypA) is bound to CA spheres with the same affinity as CA tubes but at a higher CypA/CA stoichiometry, suggesting that the level of recruitment of CypA to the HIV capsid is dependent on curvature
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Capsid Proteins
|2 NLM
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|a Cyclophilin A
|2 NLM
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| 650 |
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|a EC 5.2.1.-
|2 NLM
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| 700 |
1 |
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|a Walsh, James C
|e verfasserin
|4 aut
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1 |
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|a Mousapasandi, Amir
|e verfasserin
|4 aut
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1 |
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|a Ariotti, Nicholas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shah, Vaibhav B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Turville, Stuart
|e verfasserin
|4 aut
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1 |
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|a Jacques, David A
|e verfasserin
|4 aut
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|a Böcking, Till
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 36(2020), 13 vom: 07. Apr., Seite 3624-3632
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
1 |
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|g volume:36
|g year:2020
|g number:13
|g day:07
|g month:04
|g pages:3624-3632
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|u http://dx.doi.org/10.1021/acs.langmuir.0c00103
|3 Volltext
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|d 36
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|h 3624-3632
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