Quantification and Distribution of the 16SrIV-D Phytoplasma in the Wild Date Palm, Phoenix sylvestris, at Different Stages of Decline Using Quantitative PCR (qPCR) Analysis

Lethal bronzing disease (LBD) is a fatal infection in a variety of ornamental palms in Florida caused by the 16SrIV-D phytoplasma. The disease was first found in 2006 in Florida and has since spread to 31 different counties, reaching as far north as Jacksonville and as far south as Key Largo. Stakeh...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Plant disease. - 1997. - 104(2020), 5 vom: 02. Mai, Seite 1328-1334
1. Verfasser: Bahder, Brian W (VerfasserIn)
Weitere Verfasser: Soto, Noemi, Mou, De-Fen, Humphries, Alessandra R, Helmick, Ericka E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article Florida lethal bronzing disease palm phytoplasma qPCR
LEADER 01000naa a22002652 4500
001 NLM307887480
003 DE-627
005 20231225130543.0
007 cr uuu---uuuuu
008 231225s2020 xx |||||o 00| ||eng c
024 7 |a 10.1094/PDIS-10-19-2146-RE  |2 doi 
028 5 2 |a pubmed24n1026.xml 
035 |a (DE-627)NLM307887480 
035 |a (NLM)32202466 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Bahder, Brian W  |e verfasserin  |4 aut 
245 1 0 |a Quantification and Distribution of the 16SrIV-D Phytoplasma in the Wild Date Palm, Phoenix sylvestris, at Different Stages of Decline Using Quantitative PCR (qPCR) Analysis 
264 1 |c 2020 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 05.05.2020 
500 |a Date Revised 05.05.2020 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Lethal bronzing disease (LBD) is a fatal infection in a variety of ornamental palms in Florida caused by the 16SrIV-D phytoplasma. The disease was first found in 2006 in Florida and has since spread to 31 different counties, reaching as far north as Jacksonville and as far south as Key Largo. Stakeholders (nursery personnel, landscaping personnel, and extension agents) from across the state take and send samples for analysis. To provide better sampling recommendations and reduce the time associated with the sampling process, the distribution of the 16SrIV-D phytoplasma was examined by quantitative PCR analysis in various Phoenix sylvestris that displayed different stages of decline, including early symptoms, moderate symptoms, and late symptoms. A declining Sabal palmetto was also available for analysis and examined as well. The findings of this study revealed that regardless of the stage of decline, the highest amount of phytoplasma detected was nearest to the base of the trunk and gradually decreased further up the trunk. Also, in P. sylvestris it was found that with symptoms present, the entire trunk has a systemic infection, resulting in positive reactions for every sample taken. These data are useful to stakeholders because they provide useful sampling recommendations in that only a single sample is needed when sampling a palm suspected to be infected with phytoplasma. Also, based on these results, sampling lower on the trunk is advised 
650 4 |a Journal Article 
650 4 |a Florida 
650 4 |a lethal bronzing disease 
650 4 |a palm 
650 4 |a phytoplasma 
650 4 |a qPCR 
700 1 |a Soto, Noemi  |e verfasserin  |4 aut 
700 1 |a Mou, De-Fen  |e verfasserin  |4 aut 
700 1 |a Humphries, Alessandra R  |e verfasserin  |4 aut 
700 1 |a Helmick, Ericka E  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant disease  |d 1997  |g 104(2020), 5 vom: 02. Mai, Seite 1328-1334  |w (DE-627)NLM098181742  |x 0191-2917  |7 nnns 
773 1 8 |g volume:104  |g year:2020  |g number:5  |g day:02  |g month:05  |g pages:1328-1334 
856 4 0 |u http://dx.doi.org/10.1094/PDIS-10-19-2146-RE  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 104  |j 2020  |e 5  |b 02  |c 05  |h 1328-1334