Examination of vesicular stomatitis virus-induced morphology changes in individual Vero cells by QMod microscopy

Viral infection of cultured cells induces changes in the biophysical characteristics of the affected cells. Advanced microscopic cameras such as Ovizio's QMod, coupled with the appropriate software, can measure a variety of characteristics on a per-cell basis. We have employed this system to mo...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 68(2020), 6 vom: 03. Juni, Seite 305-310
Auteur principal: Scholz, Isabel (Auteur)
Autres auteurs: Montoya, Christopher, Vela, Eric
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:BioTechniques
Sujets:Journal Article Vero cells microscopy vesicular stomatitis virus viral vector
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520 |a Viral infection of cultured cells induces changes in the biophysical characteristics of the affected cells. Advanced microscopic cameras such as Ovizio's QMod, coupled with the appropriate software, can measure a variety of characteristics on a per-cell basis. We have employed this system to monitor the progression of vesicular stomatitis virus infection in Vero cells and to describe the cellular changes associated with advancing vesicular stomatitis virus infection. The measurements of cellular characteristics are operator-independent, and the goal is to establish a robust method to mathematically determine viral infection levels in a given sample. This will provide a means to measure viral titer in a faster and less subjective way than manual reading of plaque assays or tissue culture infectious dose 50 assays 
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