Transcriptional regulation of NIN expression by IPN2 is required for root nodule symbiosis in Lotus japonicus

Transcriptional regulation of NIN expression by IPN2 is required for root nodule symbiosis in Lotus japonicus

© 2020 The Authors. New Phytologist © 2020 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 227(2020), 2 vom: 15. Juli, Seite 513-528
1. Verfasser: Xiao, Aifang (VerfasserIn)
Weitere Verfasser: Yu, Haixiang, Fan, Yuqian, Kang, Heng, Ren, Yaping, Huang, Xiaoqin, Gao, Xiumei, Wang, Chao, Zhang, Zhongming, Zhu, Hui, Cao, Yangrong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Lotus japonicus DNA binding gene expression regulation root nodule symbiosis transcription activation Plant Proteins
Beschreibung
Zusammenfassung:© 2020 The Authors. New Phytologist © 2020 New Phytologist Trust.
Expression of Nodule Inception (NIN) is essential for initiation of legume-rhizobial symbiosis. An existing model regarding the regulation of NIN expression involves two GRAS transcription factors - NSP1 (Nodulation Signaling Pathway 1) and NSP2. NSP2 forms a complex with NSP1 to directly bind to NIN promoter. However, rhizobial treatment-induced NIN expression could still be detected in the nsp1 mutant plants, suggesting that other proteins must be involved in the regulation of NIN expression. A combination of molecular, biochemical and genetic analyses was used to investigate the molecular basis of IPN2 in regulating root development and NIN expression in Lotus japonicus. In this study, we identified that IPN2 is a close homolog of Arabidopsis APL (ALTERED PHLOEM DEVELOPMENT) with essential function in root development. However, Lotus IPN2 has a different expression pattern compared with the Arabidopsis APL gene. IPN2 binds to the IPN2-responsive cis element (IPN2-RE) of NIN promoter and activates NIN expression. IPN2, NSP1 and NSP2 form a protein complex to directly target NIN promoter and activate NIN expression in the legume-rhizobial symbiosis. Our data refine the regulatory model of NIN expression that NSP2 works together with NSP1 and IPN2 to activate the NIN gene allowing nodulation in L. japonicus
Beschreibung:Date Completed 14.05.2021
Date Revised 14.05.2021
published: Print-Electronic
GENBANK: HQ343457, XP_020223761, LOC101507545
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.16553