The HARE chip for efficient time-resolved serial synchrotron crystallography

open access.

Bibliographische Detailangaben
Veröffentlicht in:	Journal of synchrotron radiation. - 1994. - 27(2020), Pt 2 vom: 01. März, Seite 360-370
1. Verfasser:	Mehrabi, Pedram (VerfasserIn)
Weitere Verfasser:	Müller-Werkmeister, Henrike M, Leimkohl, Jan Philipp, Schikora, Hendrik, Ninkovic, Jelena, Krivokuca, Silvia, Andriček, Ladislav, Epp, Sascha W, Sherrell, Darren, Owen, Robin L, Pearson, Arwen R, Tellkamp, Friedjof, Schulz, Eike C, Miller, R J Dwayne
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	Journal of synchrotron radiation
Schlagworte:	Journal Article HARE LAMA data collection fixed-target serial synchrotron crystallography sample delivery time-resolved crystallography

Beschreibung
Zusammenfassung:	open access. Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided
Beschreibung:	Date Completed 11.03.2020 Date Revised 13.11.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE
ISSN:	1600-5775
DOI:	10.1107/S1600577520000685