Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula

Species of the genus Trichoderma are filamentous fungi commonly used in research, industry and agriculture. Trichoderma reesei strains are prominent producers of cellulolytic and hemicellulolytic enzymes as well as being expression hosts; several other species such as T. atroviride might be exploite...

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Détails bibliographiques
Publié dans:BioTechniques. - 1993. - 68(2020), 5 vom: 01. Mai, Seite 279-282
Auteur principal: Schütz, Georg (Auteur)
Autres auteurs: Haltrich, Dietmar, Atanasova, Lea
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't Trichoderma atroviride Trichoderma reesei conidia hemocytometer spectrophotometer spore concentration spore suspension
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520 |a Species of the genus Trichoderma are filamentous fungi commonly used in research, industry and agriculture. Trichoderma reesei strains are prominent producers of cellulolytic and hemicellulolytic enzymes as well as being expression hosts; several other species such as T. atroviride might be exploited as biocontrol agents. A careful preparation of Trichoderma inocula, which consists mainly of conidia (asexual spores), is of immense importance. Conidia concentration is still mostly determined with the help of a hemocytometer; however, as a more accurate and time-saving alternative, absorbance can be used to estimate fungal spore counts. We established a spectrophotometric method for fast and reliable preparation of Trichoderma inocula by evaluating the effect of size, shape and pigmentation of the conidia at different wavelengths 
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650 4 |a Research Support, Non-U.S. Gov't 
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700 1 |a Haltrich, Dietmar  |e verfasserin  |4 aut 
700 1 |a Atanasova, Lea  |e verfasserin  |4 aut 
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