Serum biochemistry of the Arrau turtle (Podocnemis expansa) Frozen analyte stability and the effects of long-term storage

Serum biochemistry of the Arrau turtle (Podocnemis expansa) : Frozen analyte stability and the effects of long-term storage

© 2020 American Society for Veterinary Clinical Pathology.

Bibliographische Detailangaben
Veröffentlicht in:Veterinary clinical pathology. - 1975. - 49(2020), 1 vom: 06. März, Seite 42-47
1. Verfasser: Camargo, Fabricio (VerfasserIn)
Weitere Verfasser: Abrão, Nadjanaira B, Queiroz, Thawanne D, Arhnold, Emmanuel, Araújo, Luciana B M, Martins, Danieli B
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Veterinary clinical pathology
Schlagworte:Journal Article blood analytes chelonian frozen samples long-term stability reptile Blood Proteins Serum Albumin Urea 8W8T17847W mehr... Cholesterol 97C5T2UQ7J gamma-Glutamyltransferase EC 2.3.2.2 Aspartate Aminotransferases EC 2.6.1.1 Phosphotransferases EC 2.7.- Alkaline Phosphatase EC 3.1.3.1 Calcium SY7Q814VUP
Beschreibung
Zusammenfassung:© 2020 American Society for Veterinary Clinical Pathology.
BACKGROUND: Turtles are a major source of protein for riverside human populations in Brazil. The encouragement of commercial breeding meets conservation efforts for these animals, and it is, therefore, crucial to understand the physiologic and behavioral aspects of semi-aquatic species in captive conditions. Serum biochemical tests are ancillary diagnostic tools, and sample storage is a main problem since clinical laboratories are not always available near the habitats of these species
OBJECTIVES: The aim of this study was to provide information about the stability of albumin, aspartate aminotransferase (AST), calcium, creatinine kinase (CK), total cholesterol (Chol), alkaline phosphatase (ALP), gamma glutamyltransferase (GGT), total protein (TP), and urea at different storage times
METHODS: In all, 17 Arrau turtles (Podocnemis expansa) were used, and the serum obtained was separated into aliquots and analyzed at 0, 4, 8, 16, and 32 days after being stored at -20°C
RESULTS: The results showed that albumin, AST, CK, GGT, and TP suffered interference due to the long storage times
CONCLUSION: Analytes such as ALP, calcium, Chol, and urea can be evaluated for up to 1 month after freezing. Albumin, AST, and TP can be analyzed up to 1 week after freezing without alterations, and CK GGT are best evaluated on fresh samples
Beschreibung:Date Completed 25.01.2021
Date Revised 25.01.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1939-165X
DOI:10.1111/vcp.12828