|
|
|
|
LEADER |
01000naa a22002652 4500 |
001 |
NLM307207617 |
003 |
DE-627 |
005 |
20231225125042.0 |
007 |
cr uuu---uuuuu |
008 |
231225s2020 xx |||||o 00| ||eng c |
024 |
7 |
|
|a 10.1093/jxb/eraa113
|2 doi
|
028 |
5 |
2 |
|a pubmed24n1023.xml
|
035 |
|
|
|a (DE-627)NLM307207617
|
035 |
|
|
|a (NLM)32129444
|
040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
041 |
|
|
|a eng
|
100 |
1 |
|
|a Wang, Chengcheng
|e verfasserin
|4 aut
|
245 |
1 |
0 |
|a OASTL-A1 functions as a cytosolic cysteine synthase and affects arsenic tolerance in rice
|
264 |
|
1 |
|c 2020
|
336 |
|
|
|a Text
|b txt
|2 rdacontent
|
337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
500 |
|
|
|a Date Completed 14.05.2021
|
500 |
|
|
|a Date Revised 14.05.2021
|
500 |
|
|
|a published: Print
|
500 |
|
|
|a Citation Status MEDLINE
|
520 |
|
|
|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
|
520 |
|
|
|a Arsenic (As) contamination in paddy soil can cause phytotoxicity and elevated As accumulation in rice grains. Arsenic detoxification is closely linked to sulfur assimilation, but the genes involved have not been described in rice. In this study, we characterize the function of OASTL-A1, an O-acetylserine(thiol) lyase, in cysteine biosynthesis and detoxification of As in rice. Tissue expression analysis revealed that OsOASTL-A1 is mainly expressed in roots at the vegetative growth stage and in nodes at the reproductive stage. Furthermore, the expression of OsOASTL-A1 in roots was strongly induced by As exposure. Transgenic rice plants expressing pOsOASTL-A1::GUS (β-glucuronidase) indicated that OsOASTL-A1 was strongly expressed in the outer cortex and the vascular cylinder in the root mature zone. Subcellular localization using OsOASTL-A1:eGFP (enhanced green fluorescent protein) fusion protein showed that OsOASTL-A1 was localized to the cytosol. In vivo and in vitro enzyme activity assays showed that OsOASTL-A1 possessed the O-acetylserine(thiol) lyase activity. Knockout of OsOASTL-A1 led to significantly lower levels of cysteine, glutathione, and phytochelatins in roots and increased sensitivity to arsenate stress. Furthermore, the osoastl-a1 knockout mutants reduced As accumulation in the roots, but increased As accumulation in shoots. We conclude that OsOASTL-A1 is the cytosolic O-acetylserine(thiol) lyase that plays an important role in non-protein thiol biosynthesis in roots for As detoxification
|
650 |
|
4 |
|a Journal Article
|
650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
650 |
|
4 |
|a O-acetylserine(thiol) lyase
|
650 |
|
4 |
|a Arsenic
|
650 |
|
4 |
|a cysteine synthase
|
650 |
|
4 |
|a phytochelatin
|
650 |
|
4 |
|a rice
|
650 |
|
7 |
|a Cysteine Synthase
|2 NLM
|
650 |
|
7 |
|a EC 2.5.1.47
|2 NLM
|
650 |
|
7 |
|a Cysteine
|2 NLM
|
650 |
|
7 |
|a K848JZ4886
|2 NLM
|
650 |
|
7 |
|a Arsenic
|2 NLM
|
650 |
|
7 |
|a N712M78A8G
|2 NLM
|
700 |
1 |
|
|a Zheng, Lihua
|e verfasserin
|4 aut
|
700 |
1 |
|
|a Tang, Zhong
|e verfasserin
|4 aut
|
700 |
1 |
|
|a Sun, Shengkai
|e verfasserin
|4 aut
|
700 |
1 |
|
|a Ma, Jian Feng
|e verfasserin
|4 aut
|
700 |
1 |
|
|a Huang, Xin-Yuan
|e verfasserin
|4 aut
|
700 |
1 |
|
|a Zhao, Fang-Jie
|e verfasserin
|4 aut
|
773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 71(2020), 12 vom: 22. Juni, Seite 3678-3689
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
773 |
1 |
8 |
|g volume:71
|g year:2020
|g number:12
|g day:22
|g month:06
|g pages:3678-3689
|
856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/eraa113
|3 Volltext
|
912 |
|
|
|a GBV_USEFLAG_A
|
912 |
|
|
|a SYSFLAG_A
|
912 |
|
|
|a GBV_NLM
|
912 |
|
|
|a GBV_ILN_350
|
951 |
|
|
|a AR
|
952 |
|
|
|d 71
|j 2020
|e 12
|b 22
|c 06
|h 3678-3689
|