|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM307201945 |
| 003 |
DE-627 |
| 005 |
20250226211335.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/vcp.12827
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1023.xml
|
| 035 |
|
|
|a (DE-627)NLM307201945
|
| 035 |
|
|
|a (NLM)32128863
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Fonseca, Leandro A
|e verfasserin
|4 aut
|
| 245 |
1 |
4 |
|a The effects of sex, body length, and different habitats on the serum protein electrophoresis of the broad-snouted caiman (Caiman latirostris)
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 25.01.2021
|
| 500 |
|
|
|a Date Revised 25.01.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2020 American Society for Veterinary Clinical Pathology.
|
| 520 |
|
|
|a BACKGROUND: Knowledge of mammalian inflammatory responses is vast; however, many aspects of the inflammatory response in non-mammalian vertebrates, such as reptiles, remain unclear, including those regarding acute-phase proteins (APPs). Recent studies have focused on the use of serum protein electrophoresis (SPE) to assess inflammatory responses in the broad-snouted caiman (Caiman latirostris) and other reptiles
|
| 520 |
|
|
|a OBJECTIVES: The purpose of this study was to examine the effects of sex, body length, and different habitats on SPE patterns in C latirostris using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
|
| 520 |
|
|
|a METHODS: A total of 40 animals of both sexes and varying body lengths were collected; of these, 23 were free-living in an industrial complex (site 1), and 17 were captive on a rural property (site 2). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed to fractionate different serum protein constituents
|
| 520 |
|
|
|a RESULTS: Sex affected protein levels, with females showing higher serum levels of total protein, as well as the 90-, 66-, and 58-kDa proteins compared with males. Similarly, body length affected protein levels, with male adults >1.70 m in length showing the lowest serum levels of 152-, 58-, 54-, and 27-kDa proteins of all other animals. Finally, habitat affected protein levels, with animals at site 1 showing higher serum levels of 152- and 41-kDa proteins compared with those at site 2
|
| 520 |
|
|
|a CONCLUSIONS: This preliminary study was the first to evaluate the SPE of C latirostris using SDS-PAGE. Further studies to identify the proteins in each band with more specific and sensitive techniques (eg, mass spectrometry) should be conducted to elucidate the standard of APPs in reptiles better
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a SDS-PAGE
|
| 650 |
|
4 |
|a protein
|
| 650 |
|
4 |
|a reptiles
|
| 650 |
|
4 |
|a serum biochemistry
|
| 650 |
|
7 |
|a Blood Proteins
|2 NLM
|
| 700 |
1 |
|
|a Dornelas, Lorraine R S M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Orozco, Andres M O
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Souto, Pollyanna C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bento, Lucas D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Nóbrega, Yhuri C
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Santos, Marcelo R D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Carvalho Filho, Wilson P
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ermita, Pedro A N
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Girardi, Fabricia M
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 49(2020), 1 vom: 15. März, Seite 59-65
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnas
|
| 773 |
1 |
8 |
|g volume:49
|g year:2020
|g number:1
|g day:15
|g month:03
|g pages:59-65
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/vcp.12827
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 49
|j 2020
|e 1
|b 15
|c 03
|h 59-65
|