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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/eraa092
|2 doi
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|a pubmed24n1023.xml
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|a (DE-627)NLM306924234
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|a (NLM)32100005
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Ludi
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen
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1 |
|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 10.05.2021
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|a Date Revised 29.01.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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| 520 |
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|a Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilization. Mechanistically, one of the best-studied SI systems is that of Papaver rhoeas (poppy). This utilizes two S-determinants: stigma-expressed PrsS and pollen-expressed PrpS. Interaction of cognate PrpS-PrsS triggers a signalling network, causing rapid growth arrest and programmed cell death (PCD) in incompatible pollen. We previously demonstrated that transgenic Arabidopsis thaliana pollen expressing PrpS-green fluorescent protein (GFP) can respond to Papaver PrsS with remarkably similar responses to those observed in incompatible Papaver pollen. Here we describe recent advances using these transgenic plants combined with genetically encoded fluorescent probes to monitor SI-induced cellular alterations, including cytosolic calcium, pH, the actin cytoskeleton, clathrin-mediated endocytosis (CME), and the vacuole. This approach has allowed us to study the SI response in depth, using multiparameter live-cell imaging approaches that were not possible in Papaver. This lays the foundations for new opportunities to elucidate key mechanisms involved in SI. Here we establish that CME is disrupted in self-incompatible pollen. Moreover, we reveal new detailed information about F-actin remodelling in pollen tubes after SI
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4 |
|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Actin
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| 650 |
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4 |
|a actin-binding proteins (ABPs)
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| 650 |
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4 |
|a calcium
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| 650 |
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4 |
|a endocytosis
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| 650 |
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4 |
|a fluorescent probes
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| 650 |
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4 |
|a live-cell imaging
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| 650 |
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4 |
|a pH
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| 650 |
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4 |
|a pollen tube growth
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| 650 |
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4 |
|a programmed cell death (PCD)
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| 650 |
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4 |
|a self-incompatibility (SI)
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Triviño, Marina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Zongcheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carli, José
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Eaves, Deborah J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Van Damme, Daniёl
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nowack, Moritz K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Franklin-Tong, Vernonica E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bosch, Maurice
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 71(2020), 8 vom: 23. Apr., Seite 2451-2463
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:71
|g year:2020
|g number:8
|g day:23
|g month:04
|g pages:2451-2463
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/eraa092
|3 Volltext
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|a SYSFLAG_A
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|a GBV_ILN_350
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|a AR
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|d 71
|j 2020
|e 8
|b 23
|c 04
|h 2451-2463
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