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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-020-02173-9
|2 doi
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|a pubmed25n1022.xml
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|a (DE-627)NLM30681742X
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|a (NLM)32088881
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhang, He-Cai
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A novel sigma class glutathione S-transferase gene in freshwater planarian Dugesia japonica
|b cloning, characterization and protective effects in herbicide glyphosate stress
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| 264 |
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|c 2020
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 31.03.2020
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| 500 |
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a As the top-selling herbicide in the world, glyphosate distributes widely in natural environment and its influence on the ecological security and human health has attracted more and more concern. Glutathione S-transferases (GSTs) are a well-characterized superfamily of isoenzymes for cellular defense against exogenous toxic substances and therefore protect organisms from injury. In this study, the complete cDNA sequence of GST gene (named as Dja-GST) in freshwater planarian Dugesia japonica was firstly cloned by means of RACE method. The full-length Dja-GST comprises of 706 nucleotides which encodes a polypeptide of 200 amino acids. Dja-GST has two representative GST domains at the N- and C-termini. The conservative GST-N domain includes G-site Y8, F9, R14, W39, K43, P52 and S64, while the variable GST-C domain contains H-site K104, V156, D159 and L161. Sequence analysis, phylogenetic tree reconstruction and multiple alignment collectively indicate that Dja-GST belongs to the Sigma class of GST superfamily. Also, GST gene expression profile, GST enzymatic activity and MDA content in response to glyphosate exposure were systematically investigated and the correlations among them were analyzed. The results suggest that glyphosate exposure modified the mRNA transcription and enzymatic activity of GST, as well as the MDA content in planarians, indicating that Dja-GST might play an important part in organisms defending against oxidative stress induced by glyphosate. This work lays a molecular foundation for further exploring the exact functions of Dja-GST and gives an important implication for evaluating the ecological environment effects of herbicide glyphosate
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4 |
|a Journal Article
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|a Dugesia japonica
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| 650 |
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4 |
|a Glutathione S-transferase
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| 650 |
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4 |
|a Glyphosate
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| 650 |
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4 |
|a Sigma GST
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| 650 |
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4 |
|a qRT-PCR
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| 650 |
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7 |
|a Herbicides
|2 NLM
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| 650 |
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7 |
|a Water Pollutants, Chemical
|2 NLM
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| 650 |
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7 |
|a Glutathione Transferase
|2 NLM
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| 650 |
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7 |
|a EC 2.5.1.18
|2 NLM
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| 650 |
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7 |
|a Glycine
|2 NLM
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| 650 |
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7 |
|a TE7660XO1C
|2 NLM
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| 700 |
1 |
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|a Yang, Yu-Juan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ma, Ke-Xue
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shi, Chang-Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Guang-Wen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, De-Zeng
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 29(2020), 3 vom: 22. Apr., Seite 295-304
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnas
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| 773 |
1 |
8 |
|g volume:29
|g year:2020
|g number:3
|g day:22
|g month:04
|g pages:295-304
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| 856 |
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|u http://dx.doi.org/10.1007/s10646-020-02173-9
|3 Volltext
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|a AR
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|d 29
|j 2020
|e 3
|b 22
|c 04
|h 295-304
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