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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-09-19-1925-RE
|2 doi
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| 028 |
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2 |
|a pubmed24n1021.xml
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zheng, Kuanyu
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Characterization of a New Orthotospovirus from Chilli Pepper in Yunnan Province, China
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| 264 |
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|c 2020
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 07.04.2020
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|a Date Revised 08.04.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Chilli pepper (Capsicum annuum L.) is one of the most important crops in Yunnan Province, China. An orthotospovirus isolate 14YV855 was isolated from a diseased chilli pepper plant exhibiting yellow ringspots and necrosis on leaves in Shiping County, Honghe Hani and Yi Autonomous Prefecture, Yunnan Province in 2014. The complete genome sequence of 14YV855 was determined. The small, medium, and large RNAs are 3,428, 4,781, and 8,917 nucleotides long, respectively. The complete nucleocapsid (N) protein of 14YV855 shares a high amino acid identity of 84.8 to 89.9% to that of Capsicum chlorosis virus (CaCV), Groundnut bud necrosis virus (GBNV), Watermelon bud necrosis virus (WBNV), and Watermelon silver mottle virus (WSMoV), which is slightly less than the 90% identity threshold for the demarcation of new Orthotospovirus sp. Phylogenetic analyses revealed that the N protein and RNA-dependent RNA polymerase of 14YV855 are the most related to WSMoV, while the NSs, NSm, and Gn/Gc proteins are similar to those of GBNV. As expected, 14YV855 is serologically related to CaCV, GBNV, WBNV, and WSMoV when the monoclonal antibody against the N protein of WSMoV was used; however, 14YV855 can be distinguished from other orthotospoviruses by reverse-transcription PCR using the specific primers. Our results indicate that 14YV855 is a new Orthotospovirus sp. belonging to the WSMoV serogroup and is provisionally named Chilli yellow ringspot virus
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a genome
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| 650 |
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4 |
|a orthotospovirus
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| 650 |
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4 |
|a phylogeny
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| 650 |
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7 |
|a RNA, Viral
|2 NLM
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| 700 |
1 |
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|a Chen, Tsung-Chi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Kuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kang, Ya-Chi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yeh, Shyi-Dong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Zhongkai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dong, Jiahong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 104(2020), 4 vom: 27. Apr., Seite 1175-1182
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
8 |
|g volume:104
|g year:2020
|g number:4
|g day:27
|g month:04
|g pages:1175-1182
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-09-19-1925-RE
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