A simple and reliable method for determination of optimum pH in coupled enzyme assays
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kina...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 68(2020), 4 vom: 01. Apr., Seite 200-203
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| 1. Verfasser: |
Bowman, Lee
(VerfasserIn) |
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| Weitere Verfasser: |
Motamed, Rachael,
Lee, Paul,
Aleem, Kadijah,
Berawala, Astha S,
Hayden, Katherine L,
Bzik, David J,
Chattopadhyay, Debasish |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Cryptosporidium parvum
Plasmodium falciparum
coupled enzyme assay
enzyme kinetics
lactate dehydrogenase
pH optimization
pyruvate kinase
L-Lactate Dehydrogenase
mehr...
EC 1.1.1.27
Pyruvate Kinase
EC 2.7.1.40 |
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