Molecular characterization of three caspases from Bostrychus sinensis and their transcriptional responses to bacteria and viruses
© 2020 John Wiley & Sons Ltd.
| Publié dans: | Journal of fish diseases. - 1998. - 43(2020), 4 vom: 13. Apr., Seite 431-443 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2020
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| Accès à la collection: | Journal of fish diseases |
| Sujets: | Journal Article Vibrio parahaemolyticus Chinese black sleeper caspase expression analysis poly(I:C) Fish Proteins Caspases EC 3.4.22.- Poly I-C |
| Résumé: | © 2020 John Wiley & Sons Ltd. The caspase family proteins are aspartate-specific cysteine proteases that transmit extracellular signals to cells, ultimately cause apoptosis and therefore play a key role in cellular immunity. In this study, we cloned and characterized three caspases from Chinese black sleeper (Bostrychus sinensis), Bscasp-1, Bscasp-8 and Bscasp-9. Real-time PCR analysis showed that Bscasp-1, Bscasp-8 and Bscasp-9 were universally expressed in all tested tissues of B. sinensis. Expression analyses showed that after poly(I:C) stimulation and bacterial (Vibrio parahaemolyticus) infection, the three caspases were significantly upregulated. After poly(I:C) stimulation, the change of Bscasp-1 expression in the head kidney was the most obvious; peak expression was about 80.78-fold more than that of the control. In addition, the expression of Bscasp-8 and Bscasp-9 in the peripheral blood and liver was 167.99- and 17.98-fold higher than that in the control group, respectively. After V. parahaemolyticus infection, the expression peaks of Bscasp-1 and Bscasp-8 in the peripheral blood and spleen were 85.82-fold and 280.83-fold that of the control. However, the expression of Bscasp-9 in the peripheral blood was upregulated only 8.33-fold higher than that in the control group. These results indicate that Bscasp-1, Bscasp-8 and Bscasp-9 are likely involved in response to viral and bacterial infection |
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| Description: | Date Completed 11.09.2020 Date Revised 30.09.2020 published: Print-Electronic GENBANK: MN646911, MN646912, MN646913, XP_020784268.1, XP_028429215.1, AHG06617.1, AUO15551.1, NP_571580.1, XP_020795173.1, ARM65447.1, AVD97789.1, QCB64752.1, XP_023154014.1, XP_005165894.1, XP_023132027.1, AHG06616.1, XP_010753774.3, XP_028264730.1, XP_025757561.1, AXE71620.1, NP_001007405.2 Citation Status MEDLINE |
| ISSN: | 1365-2761 |
| DOI: | 10.1111/jfd.13140 |