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|a eng
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1 |
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|a Wei, Shan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
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|c 2020
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|a Text
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|a ƒaComputermedien
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|a Date Completed 11.10.2021
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|a Date Revised 28.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications
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|a Research Support, Non-U.S. Gov't
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|a genetic testing
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|a rapid clinical diagnostics
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| 650 |
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|a rapid tissue gDNA extraction
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| 650 |
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|a tissue processing
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|a DNA
|2 NLM
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| 700 |
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|a Levy, Brynn
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|a Hoffman, Nataly
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1 |
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1 |
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|a Rodney-Sandy, Reunet
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| 700 |
1 |
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|a Wapner, Ronald
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| 700 |
1 |
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|a D'Alton, Mary
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|4 aut
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| 700 |
1 |
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|a Williams, Zev
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 68(2020), 5 vom: 13. Mai, Seite 240-244
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|x 1940-9818
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