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|a 10.1094/PDIS-02-19-0353-RE
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|a eng
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|a Avenot, Hervé F
|e verfasserin
|4 aut
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|a Resistance to Thiophanate-Methyl in Botrytis cinerea Isolates From Californian Vineyards and Pistachio and Pomegranate Orchards
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|c 2020
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|a Text
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|a ƒaComputermedien
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|a Date Completed 07.04.2020
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|a Date Revised 08.04.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In this study, a mycelial growth assay was used to evaluate the sensitivity to thiophanate-methyl of 144 Botrytis cinerea isolates (collection A) from Californian vineyards and pistachio and pomegranate orchards. Based on the effective concentration that inhibits 50% of growth (EC50) values for mycelial growth inhibition on fungicide-amended media, 3, 28, 10, and 58% of the isolates showed sensitivity (SS; EC50 < 1 µg/ml), low resistance (LR; 1 < EC50 < 10 µg/ml), weak resistance (WR; 10 < EC50 < 50 µg/ml), and high resistance (HR; EC50 > 100 µg/ml) toward thiophanate-methyl, respectively. The LR and HR phenotypes were observed in pistachio and pomegranate orchards, even though pomegranate was not sprayed with thiophanate-methyl. Sensitivity to thiophanate-methyl of a historical collection of 257 B. cinerea isolates (collection B) isolated from pistachio orchards in 1992, 2005, and 2006 was assessed on potato dextrose agar amended with thiophanate-methyl at the discriminatory concentration of 10 µg/ml. Average percentages of thiophanate-methyl-resistant isolates were 50, 72, and 64% in the orchards in 1992, 2005, and 2006, respectively. A study of fitness components of selected thiophanate-methyl-resistant (LR, WR, and HR) and -sensitive (SS) isolates from collection A did not reveal any significant difference between them with respect to mycelial growth on fungicide-free media and pathogenicity on cultivar Crimson Seedless berries. Comparison of β-tubulin sequences from resistant and sensitive phenotypes revealed that a glutamic acid at position 198 was changed to alanine in all HR isolates and three LR isolates. The occurrence of thiophanate-methyl resistance in B. cinerea populations should be considered when designing spray programs against blossom and shoot blight of pistachio and gray mold of grape
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|a Journal Article
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|a chemical
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|a disease management
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|a fruit
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|a fungi
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|a tree fruits
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|a tree nuts
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|a trees
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|a Thiophanate
|2 NLM
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| 650 |
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|2 NLM
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| 700 |
1 |
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|a Morgan, David P
|e verfasserin
|4 aut
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| 700 |
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|a Quattrini, Joel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Michailides, Themis J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 104(2020), 4 vom: 01. Apr., Seite 1069-1075
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|g pages:1069-1075
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