Combined GWAS and eQTL analysis uncovers a genetic regulatory network orchestrating the initiation of secondary cell wall development in cotton

Combined GWAS and eQTL analysis uncovers a genetic regulatory network orchestrating the initiation of secondary cell wall development in cotton

© 2020 The Authors. New Phytologist © 2020 New Phytologist Trust.

Détails bibliographiques
Publié dans:The New phytologist. - 1984. - 226(2020), 6 vom: 15. Juni, Seite 1738-1752
Auteur principal: Li, Zhonghua (Auteur)
Autres auteurs: Wang, Pengcheng, You, Chunyuan, Yu, Jiwen, Zhang, Xiangnan, Yan, Feilin, Ye, Zhengxiu, Shen, Chao, Li, Baoqi, Guo, Kai, Liu, Nian, Thyssen, Gregory N, Fang, David D, Lindsey, Keith, Zhang, Xianlong, Wang, Maojun, Tu, Lili
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:The New phytologist
Sujets:Journal Article Research Support, Non-U.S. Gov't cell elongation cell wall synthesis cotton fibre eQTL genetic regulation
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520 |a The cotton fibre serves as a valuable experimental system to study cell wall synthesis in plants, but our understanding of the genetic regulation of this process during fibre development remains limited. We performed a genome-wide association study (GWAS) and identified 28 genetic loci associated with fibre quality in allotetraploid cotton. To investigate the regulatory roles of these loci, we sequenced fibre transcriptomes of 251 cotton accessions and identified 15 330 expression quantitative trait loci (eQTL). Analysis of local eQTL and GWAS data prioritised 13 likely causal genes for differential fibre quality in a transcriptome-wide association study (TWAS). Characterisation of distal eQTL revealed unequal genetic regulation patterns between two subgenomes, highlighted by an eQTL hotspot (Hot216) that established a genome-wide genetic network regulating the expression of 962 genes. The primary regulatory role of Hot216, and specifically the gene encoding a KIP-related protein, was found to be the transcriptional regulation of genes responsible for cell wall synthesis, which contributes to fibre length by modulating the developmental transition from rapid cell elongation to secondary cell wall synthesis. This study uncovered the genetic regulation of fibre-cell development and revealed the molecular basis of the temporal modulation of secondary cell wall synthesis during plant cell elongation 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a cell elongation 
650 4 |a cell wall synthesis 
650 4 |a cotton fibre 
650 4 |a eQTL 
650 4 |a genetic regulation 
700 1 |a Wang, Pengcheng  |e verfasserin  |4 aut 
700 1 |a You, Chunyuan  |e verfasserin  |4 aut 
700 1 |a Yu, Jiwen  |e verfasserin  |4 aut 
700 1 |a Zhang, Xiangnan  |e verfasserin  |4 aut 
700 1 |a Yan, Feilin  |e verfasserin  |4 aut 
700 1 |a Ye, Zhengxiu  |e verfasserin  |4 aut 
700 1 |a Shen, Chao  |e verfasserin  |4 aut 
700 1 |a Li, Baoqi  |e verfasserin  |4 aut 
700 1 |a Guo, Kai  |e verfasserin  |4 aut 
700 1 |a Liu, Nian  |e verfasserin  |4 aut 
700 1 |a Thyssen, Gregory N  |e verfasserin  |4 aut 
700 1 |a Fang, David D  |e verfasserin  |4 aut 
700 1 |a Lindsey, Keith  |e verfasserin  |4 aut 
700 1 |a Zhang, Xianlong  |e verfasserin  |4 aut 
700 1 |a Wang, Maojun  |e verfasserin  |4 aut 
700 1 |a Tu, Lili  |e verfasserin  |4 aut 
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