Molecular and functional characterization of the SBP-box transcription factor SPL-CNR in tomato fruit ripening and cell death

Molecular and functional characterization of the SBP-box transcription factor SPL-CNR in tomato fruit ripening and cell death

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 71(2020), 10 vom: 30. Mai, Seite 2995-3011
1. Verfasser: Lai, Tongfei (VerfasserIn)
Weitere Verfasser: Wang, Xiaohong, Ye, Bishun, Jin, Mingfei, Chen, Weiwei, Wang, Ying, Zhou, Yingying, Blanks, Andrew M, Gu, Mei, Zhang, Pengcheng, Zhang, Xinlian, Li, Chunyang, Wang, Huizhong, Liu, Yule, Gallusci, Philippe, Tör, Mahmut, Hong, Yiguo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Colourless non-ripening Cell death SlSPL-CNR SlSnRK1 nuclear localization signal tomato (Solanum lycopersicum) fruit ripening zinc-finger motif Ethylenes mehr... Plant Proteins Transcription Factors
Beschreibung
Zusammenfassung:© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
SlSPL-CNR, an SBP-box transcription factor (TF) gene residing at the epimutant Colourless non-ripening (Cnr) locus, is involved in tomato ripening. This epimutant provides a unique model to investigate the (epi)genetic basis of fruit ripening. Here we report that SlSPL-CNR is a nucleus-localized protein with a distinct monopartite nuclear localization signal (NLS). It consists of four consecutive residues ' 30KRKR33' at the N-terminus of the protein. Mutation of the NLS abolishes SlSPL-CNR's ability to localize in the nucleus. SlSPL-CNR comprises two zinc-finger motifs (ZFMs) within the C-terminal SBP-box domain. Both ZFMs contribute to zinc-binding activity. SlSPL-CNR can induce cell death in tomato and tobacco, dependent on its nuclear localization. However, the two ZFMs have differential impacts on SlSPL-CNR's induction of severe necrosis or mild necrotic ringspot. NLS and ZFM mutants cannot complement Cnr fruits to ripen. SlSPL-CNR interacts with SlSnRK1. Virus-induced SlSnRK1 silencing leads to reduction in expression of ripening-related genes and inhibits ripening in tomato. We conclude that SlSPL-CNR is a multifunctional protein that consists of a distinct monopartite NLS, binds to zinc, and interacts with SlSnRK1 to affect cell death and tomato fruit ripening
Beschreibung:Date Completed 14.05.2021
Date Revised 07.12.2022
published: Print
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/eraa067