An improved shotgun antisense method for mutagenesis and gene identification
Shotgun expression of antisense cDNA, where each transformed cell expresses a different antisense cDNA, has been used for mutagenesis and gene identification in Dictyostelium discoideum. However, the method has two limitations. First, there were too few clones in the shotgun antisense cDNA library t...
| Veröffentlicht in: | BioTechniques. - 1993. - 68(2020), 3 vom: 16. März, Seite 163-165 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2020
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, N.I.H., Extramural cDNA normalization genetic screen shotgun antisense DNA, Antisense DNA, Complementary DNA, Protozoan |
| Online verfügbar |
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