Revisiting Australian Ectocarpus subulatus (Phaeophyceae) From the Hopkins River : Distribution, Abiotic Environment, and Associated Microbiota
© 2020 Phycological Society of America.
| Veröffentlicht in: | Journal of phycology. - 1966. - 56(2020), 3 vom: 01. Juni, Seite 719-729 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2020
|
| Zugriff auf das übergeordnete Werk: | Journal of phycology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Ectocarpus subulatus distribution freshwater colonization low salinity adaptation microbiota |
| Zusammenfassung: | © 2020 Phycological Society of America. In 1995 a strain of Ectocarpus was isolated from Hopkins River Falls, Victoria, Australia, constituting one of few available freshwater or nearly freshwater brown algae, and the only one belonging to the genus Ectocarpus. It has since been used as a model to study acclimation and adaptation to low salinities and the role of its microbiota in these processes. To provide more background information on this model, we assessed if Ectocarpus was still present in the Hopkins river 22 years after the original finding, estimated its present distribution, described its abiotic environment, and determined its in situ microbial composition. We sampled for Ectocarpus at 15 sites along the Hopkins River as well as 10 neighboring sites and found individuals with ITS and cox1 sequences identical to the original isolate at three sites upstream of Hopkins River Falls. The salinity of the water at these sites ranged from 3.1 to 6.9, and it was rich in sulfate (1-5 mM). The diversity of bacteria associated with the algae in situ (1312 operational taxonomic units) was one order of magnitude higher than in previous studies of the original laboratory culture, and 95 alga-associated bacterial strains were isolated from algal filaments on site. In particular, species of Planctomycetes were abundant in situ but rare in laboratory cultures. Our results confirmed that Ectocarpus was still present in the Hopkins River, and the newly isolated algal and bacterial strains offer new possibilities to study the adaptation of Ectocarpus to low salinity and its interactions with its microbiome |
|---|---|
| Beschreibung: | Date Completed 19.11.2020 Date Revised 09.01.2024 published: Print-Electronic GENBANK: LR735221, LR735414, LR735222, LR735415, LR735223, LR735416, LR735224, LR735417, LR735225, LR735418, LR735226, LR735227, LR735444, LR735537 Citation Status MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/jpy.12970 |