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DE-627 |
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20231225121444.0 |
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cr uuu---uuuuu |
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231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1094/PDIS-09-19-1958-SC
|2 doi
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| 028 |
5 |
2 |
|a pubmed24n1018.xml
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|a (DE-627)NLM305563025
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| 035 |
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|a (NLM)31958249
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Alabi, Olufemi J
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A Description of the Possible Etiology of the Cilantro Yellow Blotch Disease
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| 264 |
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1 |
|c 2020
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 05.03.2020
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| 500 |
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|a Date Revised 05.03.2020
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| 500 |
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a A virus-like disease characterized by foliar yellow blotch symptoms and resembling those described for cilantro yellow blotch disease in California was observed in a 4.05-ha cilantro (Coriandrum sativum) cv. Santo field in Hidalgo County, Texas during spring 2019. Disease incidence at harvest was estimated at ∼20%, and the affected plants were rendered unmarketable. Foliar systemic chlorosis symptoms were observed on sap-inoculated Nicotiana occidentalis plants (n = 3) using inocula from symptomatic cilantro. Total RNA aliquots from 11 randomly collected leaf tissue samples (symptomatic = 7, asymptomatic = 4) were pooled into a composite cilantro RNA sample which was analyzed by high throughput sequencing (HTS). Analyses of the obtained 15.7 million raw reads (76 nt each) yielded virus-specific contigs that mapped to the genomes of alfalfa mosaic virus (AMV), beet pseudoyellows virus (BPYV), and lettuce chlorosis virus (LCV). Virus-specific primers designed from the HTS-derived sequences were used to screen the samples in two-step RT-PCR assays, resulting in the detection of AMV+BPYV in 3 of 7 symptomatic cilantro samples, AMV+LCV in 4 of 7 symptomatic cilantro samples, and AMV alone in the 4 asymptomatic cilantro and sap-inoculated N. occidentalis samples. The results represent the first reports of the natural infection of cilantro by BPYV and LCV and implicate the mixed infection of a Crinivirus and AMV in cilantro yellow blotch disease
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Alfalfa mosaic virus
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| 650 |
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4 |
|a Beet pseudoyellow virus
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| 650 |
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4 |
|a Crinivirus
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| 650 |
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4 |
|a Lettuce chlorosis virus
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| 650 |
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4 |
|a alternative host
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| 650 |
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4 |
|a aphids
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| 650 |
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4 |
|a cilantro yellow blotch
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| 650 |
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4 |
|a etiology
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| 650 |
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4 |
|a mixed virus infections
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| 650 |
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4 |
|a whitefly
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| 700 |
1 |
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|a Gaytán, Brianna C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Al Rwahnih, Maher
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Villegas, Cecilia
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 104(2020), 3 vom: 21. März, Seite 630-633
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
8 |
|g volume:104
|g year:2020
|g number:3
|g day:21
|g month:03
|g pages:630-633
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS-09-19-1958-SC
|3 Volltext
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|d 104
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|e 3
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|h 630-633
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