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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.9b03793
|2 doi
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|a pubmed24n1018.xml
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|a (DE-627)NLM305496905
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|a (NLM)31951413
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Schäfer, Jonas
|e verfasserin
|4 aut
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| 245 |
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|a Leaflet-Dependent Distribution of PtdIns[4,5]P2 in Supported Model Membranes
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|c 2020
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 09.07.2020
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|a Date Revised 09.07.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Supported planar lipid bilayers (SLBs) prepared by spreading of unilamellar vesicles on hydrophilic substrates such as silicon dioxide are frequently used to investigate lipid-protein interactions by means of surface-sensitive methods. In recent years, the receptor lipid phosphatidylinositol-4,5-bisphosphate (PtdIns[4,5]P2) became particularly important as a significant number of proteins bind to this lipid at the inner leaflet of the plasma membrane. Here, we investigated how the lipid PtdIns[4,5]P2 distributes between the two leaflets of an SLB on SiO2 surfaces. We prepared SLBs on SiO2 by spreading small unilamellar vesicles and quantified the adsorption of PtdIns[4,5]P2 binding proteins providing information about the accessibility of PtdIns[4,5]P2. We compared protein binding to PtdIns[4,5]P2 in SLBs with that in lipid monolayers on a 1,1,1-trimethyl-N-(trimethylsilyl)silanamine-functionalized SiO2 surface using reflectometric interference spectroscopy and atomic force microscopy. Our results clearly demonstrate that the accessibility of PtdIns[4,5]P2 for protein binding is reduced in SLBs compared to that in supported hybrid membranes, which is discussed in terms of PtdIns[4,5]P2 distribution in the two leaflets of SLBs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Nehls, Jessica
|e verfasserin
|4 aut
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1 |
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|a Schön, Markus
|e verfasserin
|4 aut
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1 |
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|a Mey, Ingo
|e verfasserin
|4 aut
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|a Steinem, Claudia
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 36(2020), 5 vom: 11. Feb., Seite 1320-1328
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:36
|g year:2020
|g number:5
|g day:11
|g month:02
|g pages:1320-1328
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|u http://dx.doi.org/10.1021/acs.langmuir.9b03793
|3 Volltext
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|d 36
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