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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1002/adma.201906475
|2 doi
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|a DE-627
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|a eng
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|a Hou, Shuai
|e verfasserin
|4 aut
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|a A Self-Assembled Plasmonic Substrate for Enhanced Fluorescence Resonance Energy Transfer
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|c 2020
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 18.11.2020
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|a Date Revised 18.11.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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|a Fluorescence resonance energy transfer (FRET) has found widespread uses in biosensing, molecular imaging, and light harvesting. Plasmonic metal nanostructures offer the possibility of engineering photonic environment of specific fluorophores to enhance the FRET efficiency. However, the potential of plasmonic nanostructures to enable tailored FRET enhancement on planar substrates remains largely unrealized, which are of considerable interest for high-performance on-surface bioassays and photovoltaics. The main challenge lies in the necessitated concurrent control over the spectral properties of plasmonic substrates to match that of fluorophores and the fluorophore-substrate spacing. Here, a self-assembled plasmonic substrate based on polydopamine (PDA)-coated plasmonic nanocrystals is developed to effectively address this challenge. The PDA coating not only drives interfacial self-assembly of the nanocrystals to form closely packed arrays with customized optical properties, but also can serve as a tailored nanoscale spacer between the fluorophores and plasmonic nanocrystals, which collectively lead to optimized fluorescence enhancement. The biocompatible plasmonic substrate that allows convenient bioconjugation imparted by PDA has afforded improved FRET efficiency in DNA microarray assay and FRET imaging of live cells. It is envisioned that the self-assembled plasmonic substrates can be readily integrated into fluorescence-based platforms for diverse biomedical and photoconversion applications
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|a Journal Article
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|a fluorescence enhancement
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|a fluorescence resonance energy transfer
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|a live cell imaging
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|a microarray assay
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|a plasmonic substrates
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|a Biocompatible Materials
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|a polydopamine
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|a Chen, Yonghao
|e verfasserin
|4 aut
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|a Lu, Derong
|e verfasserin
|4 aut
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|a Xiong, Qirong
|e verfasserin
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|a Lim, Yun
|e verfasserin
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|a Duan, Hongwei
|e verfasserin
|4 aut
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 32(2020), 8 vom: 01. Feb., Seite e1906475
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnas
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|g volume:32
|g year:2020
|g number:8
|g day:01
|g month:02
|g pages:e1906475
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|u http://dx.doi.org/10.1002/adma.201906475
|3 Volltext
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